The Journal of Neuroscience, April 5, 2006, ():
Inhibition of Apoptosis by P2Y2 Receptor Activation: Novel Pathways for Neuronal Survival
J. Neurosci. Arthur et al.
26: 3798
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1: ATPγS-mediated inhibition of serum-starvation-induced apoptosis does not require PKC or Ca2+ in PC12 cells. Serum-starved PC12 cells and were treated with 1然 Ro 31-8220 (PKC inhibitor) and 10然 ATPγS. Cells were analyzed for apoptosis by quantitation of DNA fragmentation (A). Serum-starved PC12 cells were treated with the calcium chelator 50然 BAPTA-AM and 10然 ATPγS. Cells were analyzed for apoptosis by quantitation of DNA fragmentation (A).
- supplemental material
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Supplemental Figure 2: Extracellular NGF concentration of DRG cultures is not increased by serum deprivation or ATPγS. DRG cultures were grown for 72h and then maintained with serum or serum-deprived for 12 h with no addition, 10然 histamine or 10然 ATPγS. NGF concentration in the media was measured by ELISA. **p<0.01 vs. serum-deprived with no addition.
