The Journal of Neuroscience, April 12, 2006, ():
Semaphorin-1a Functions as a Guidance Receptor in the Drosophila Visual System
J. Neurosci. Cafferty et al.
26: 3999
Supplemental data
Files in this Data Supplement:
- supplemental material -
Supplemental table
- supplemental material
-
Supplementary Figure 1. Interfering with Plexin A signaling did not affect R-cell axon termination pattern. A, Wild type. B, A sema1aP1 hemizygote. C, R-cell termination pattern appeared largely normally in a MICAL eye-specific mosaic individual in which almost entire eye tissues were homozygous MICAL mutant cells (n=33). D, Overexpression of Nervy under control of GMR-GAL4 in R-cell axons did not affect R-cell axon termination pattern (n=10). E, Overexpression of Nervy in lamina glial cells under control of the glial-specific driver Repo-GAL4 did not affect R-cell axon termination pattern either (n=15). Scale bar: 20 μm.
- supplemental material
-
Supplementary Figure 2. The effect of expressing wild-type Sema1a and cytoplasmic-domain-truncated Sema1a in wild-type larvae under control of the neuronal-specific elav-GAL4 driver on R-cell axonal projections. A, wild type. B, Overexpression of Sema1a in wild-type larvae under control of GMR-GAL4 induced the hyper-fasciculation of R-cell axons. C, Overexpression of Sema1a in wild-type larvae under control of elav-GAL4 caused a weaker R-cell axonal hyper-fasciculation phenotype (n=6). The bundles in C were thinner than that in B. D, No defect was observed in wild-type larvae expressing the cytoplasmic-domain-truncated Sema1a under control of elav-GAL4 (n=9). Scale bar: 20 μm.
