The Journal of Neuroscience, April 12, 2006, ():
Seizures Accelerate Functional Integration of Adult-Generated Granule Cells
J. Neurosci. Overstreet-Wadiche et al.
26: 4095
Supplemental data
Files in this Data Supplement:
- supplemental material
-
Supplemental Figure 1. Biocytin-filled newborn granule cell after seizures.
The synaptic currents illustrated in Fig. 3a were recorded in this biocytin-filled newborn cell (red). Recording was done 14 days after seizure induction. Co-localization with EGFP (green) is indicated by yellow. Scale bar, 20 µm.
- supplemental material
-
Supplemental Figure 2. Hilar stimulation evokes EPSCs in newborn granule cells after seizures.
Stimulation in the hilus evoked EPSCs in 3/5 newborn granule cells. Evoked responses had long latencies and small amplitudes, similar to what was seen in newborn granule cells with exclusively recurrent MPP input (Fig. 5c). The appearance of unclamped action currents in some cells confirmed stimulation of the mossy fiber pathway (not shown). These results are consistent with the idea that polysynaptic input to newborn granule cells is generated by recurrent mossy fiber collaterals, although a contribution by mossy cell collaterals cannot be ruled out.
- supplemental material
-
Supplemental Figure 3. Basal dendrites on newborn granule cells.
Examples of two newborn granule cells filled with biocytin (yellow), six days after seizure induction. The upper cell had prominent recurrent basal dendrites with spine-like structures that were not observed in controls. Other EGFP-labeled newborn granule cells with recurrent basal dendrites are also visible. Scale bar, 40 µm.
