The Journal of Neuroscience, April 19, 2006, ():
Mild Hypoxia Promotes Survival and Proliferation of SOD2-Deficient Astrocytes via c-Myc Activation
J. Neurosci. Liu et al.
26: 4329
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. WT astrocyte culture under "normal" conditions (N) and 5% O2 hypoxia (5%) with DAPI (blue) and HEt (red) for ROS production. Less HEt staining is observed in both normoxia and 5% O2 suggesting ROS production is low. Magnification is 400×.
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Supplemental Figure 2. Effect of the antioxidant, PBN, on cell viability in SOD2 / astrocytes under normoxia (A) and 5% O2 (B). SOD2 / astrocytes were treated with 100 μM PBN and cell viability was assessed by lactate dehydrogenase assay (LDH) release. Relative LDH release as percentage of control is shown in the graphs. In Fig. 2A, there is a significant decrease in LDH release in PBN-treated astrocytes compared to non-treated astrocytes(*p < 0.05; n = 3). con, control; HYP, hypoxia.
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Supplemental Figure 3. Schematic diagram showing the cascade of the cell cycle proteins. SOD2-/- astrocytes under 5% O2 hypoxia could trigger this cascade, leading to cell survival and proliferation.
