The Journal of Neuroscience, April 26, 2006, ():
Reelin Deficiency and Displacement of Mature Neurons, But Not Neurogenesis, Underlie the Formation of Granule Cell Dispersion in the Epileptic Hippocampus
J. Neurosci. Heinrich et al.
26: 4701
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1
Schedule of the BrdU injection protocol.
After KA injection BrdU was intraperitoneally administered following two different protocols. In the first group, mice received BrdU injections, four times a day at six and seven days after KA injection (Group 1) and were sacrificed either ten days or five weeks after KA injection. In the second group, mice were given, over seven consecutive days, twice daily BrdU injections, starting eight days after KA administration, and were sacrificed after the last BrdU injection corresponding to fourteen days survival time (Group 2).
- supplemental material
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Supplementary Figure 2
Differential transcriptional responses after KA injection. Sequential hippocampal sections were processed for in situ hybridization to localize synapsin I (a, b), reelin (c, d), and BDNF (e, f) mRNAs at two days after KA injection.
a, c, e: contralateral side; b, d, f: ipsilateral side.
a, b. Equal expression of synapsin I mRNA in the contra- and ipsilateral granule cell layer. Note the ipsilateral loss of hybridization signal in the hilus which most likely reflects the death of hilar neurons.
c, d. Reelin mRNA expression is almost completely lost in the ipsilateral dentate (d) compared to the contralateral side (c).
e, f. A massive increase in BDNF mRNA expression can be observed in the granule cell layer after KA injection (f) when compared to the contralateral side (e).
GCL, granule cell layer; H, hilus. Scale bar: a – f: 75 µm.
- supplemental material
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Supplementary Figure 3
Immunostaining for doublecortin (DCX) in the ventral hippocampus six weeks after KA injection.
a, c. Contralateral side. b, d. Ipsilateral side. On both sides, many DCX-positive cells are located in the subgranular layer of the dentate gyrus (arrows in c and d). On the ipsilateral side no granule cell dispersion and no loss of DCX-positive cells can be seen.
GCL, granule cell layer. Scale bars: a, b: 200 µm; c, d: 100 µm.
