The Journal of Neuroscience, May 3, 2006, ():
Antibodies against the NG2 Proteoglycan Promote the Regeneration of Sensory Axons within the Dorsal Columns of the Spinal Cord
J. Neurosci. Tan et al.
26: 4729
Supplemental data
Files in this Data Supplement:
- supplemental material
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Figure 1S: Chondroitinase ABC infused via gelfoam promotes mechanosensory axon regeneration into the spinal cord lesion cavity. A) Chondroitinase ABC treatment: Sensory axons (CTB-labeled, red fluorescence) grow into and past the lesion cavity as demarcated by GFAP-immunoreactivity. B) Control penicillinase treatment: Labeled axons stop caudal to the lesion cavity. C and D) To determine whether the applied chondroitinase ABC entered the injury site and degraded GAG chains, lesioned tissue was stained with anti-chondroitin-4-sulfate (ΔDi-4s) antibodies. C) Immunoreactivity for ΔDi-4s was found throughout the border of the lesion cavity 7-days after injury and treatment with chondroitinase ABC treatment. D) No anti-ΔDi-4s immunoreactivity was seen after penicillinase control infusion. E and F) Neurolucida reconstructions, see Materials and Methods, of sagital sections through the dorsal columns show the extent of fiber growth in a chondroitinase ABC and a penicillinase treated animal. G) To compare the magnitude of regeneration between neutralizing anti-NG2 monoclonal antibody and chondroitinase ABC treatments at 7-days post-lesion, axon density measurements were performed as described in Materials and Methods. No statistically significant differences were found at any distances from the lesion center between anti-NG2 (n=7) and chondroitinase treatment (n=6) (p≥0.08-1.0, one-way ANOVA with Bonferonni post hoc tests). All scale bars = 500um.
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3-dimensional rendering of the reconstructed pattern of axon growth after either antibody treatment or a peripheral nerve conditioning lesion and antibody treatment. Each movie rotates the reconstructions to better visualize the patterns of axonal regeneration.
movie #1: 7 day treatment with non-neutralizing antibodies.
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movie #2: 7 day treatment with neutralizing antibodies.
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movie #3: Peripheral nerve lesion, non-neutralizing antibody treatment, 28 day survival.
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movie #4: Peripheral nerve lesion, neutralizing antibody treatment, 28 day survival.
