The Journal of Neuroscience, May 3, 2006, ():
Localization of Diacylglycerol Lipase-
around Postsynaptic Spine Suggests Close Proximity between Production Site of an Endocannabinoid, 2-Arachidonoyl-glycerol, and Presynaptic Cannabinoid CB1 Receptor
J. Neurosci. Yoshida et al.
26: 4740
Supplemental data
Files in this Data Supplement:
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Supplemental Fig. S1. The specificity of PKCγ antibody and immunohistochemistry and its distribution in Purkinje cells. A, Immunoblot with brain homogenates from wild-type (WT) and PKCγ-knockout (KO) mice. B, C, Immunofluorescence for parasagittal brain sections from wild-type and PKCγ- KO mice. D, Immunofluorescence labeling in cerebellar Purkinje cells. PKCγ is distributed in the soma, proximal dendrites, spiny branchlets, and spines (inset). E, F, Silver-enhanced preembedding immunogold showing the distribution of PKCγ in proximal dendrites (PD), and spiny branchlets (SB), and spines (Sp) of Purkinje cells. Note cell membrane-associated and cytoplasmic distributions of PKCγ. Also note its localization in both the head and neck of spines contacting to parallel fiber terminals (PF). Arrows indicate the dendrite-spine border Scale bars: B, C, 1mm; D, 20 μm, inset, 5 μm; E, F, 0.5 μm.
