The Journal of Neuroscience, May 10, 2006, ():
Dynamics of Spontaneous Activity in the Fetal Macaque Retina during Development of Retinogeniculate Pathways
J. Neurosci. Warland et al.
26: 5190
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Material 1. Movies representing the firing rate at three developmental ages: E60, E67 and E76. The multi-electrode recordings were made using an 8x8 rectangular array of electrodes (see Methods). For each cell recorded, the normalized firing rate is represented as a gray value in the corresponding element of the 8x8 matrix in the movie. Each frame corresponds to the average firing rate in a 100ms window. The duration of the entire movie is corresponds to 10 minutes in real time.
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Supplemental movie
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Supplemental movie
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Supplementary Material 2. Additional examples (similar to Figure 2 in the text) of the total activity histograms at four developmental ages: E60, E67, E71, and E76. Units of histogram are cell-spikes/sec.
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Supplementary Material 3. Additional examples (similar to Figure 3 in the text) of iso-rate surfaces of wave events in an E60 retina. The firing rates are represented as a volume where the x and y axes encode the spatial position of the cells on the array, and the z-axis encodes the time a cell fires a spike. We then plot the iso-rate surface corresponding to 25% of the maximal firing rate of the cells in this volume.
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Supplementary Material 4. Confocal photomicrographs of a retinal section from an E60 macaque, stained with DAPI to reveal cell nuclei. (A) Low power image of retina parallel to the optic nerve (ON) - temporal axis. Asterisk denotes the depression near the optic nerve. Scale bar=200μm. Dashed boxes correspond to the higher magnification images shown in the panels that follow. (B, C, D) Higher power images of the E60 retina taken from the (B) central, (C) middle, and (D) peripheral (temporal) retina. Comparison of these three panels reveal that cell density is relatively uniform across the ON-temporal axis. (B-D) Scale bar=50μm. INL, inner nuclear layer; GCL, ganglion cell layer.
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Supplementary Material 5. Spatial distribution of wave initiation sites for developmental age E60. Wave events are first identified by the total activity crossing a threshold. The next one second of activity is then averaged in time to get a spatial distribution of the activity across the array. The ‘center of mass’ of this activity image is then plotted as an ‘X’. The locations of the active electrodes are shown as red dots.
