The Journal of Neuroscience, January 11, 2006, ():
Functional Dissection of a Neuronal Network Required for Cuticle Tanning and Wing Expansion in Drosophila
J. Neurosci. Luan et al.
26: 573
Supplemental data
Files in this Data Supplement:
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Supplemental Figure 1. Gal80 is selectively expressed in NCCAP neurons in flies bearing the CCAP-Gal80 transgene. Confocal images show the pharate adult nervous system of a fly bearing two copies of the CCAP-Gal80 transgene labeled with antibodies to both Gal80 (green) and the bursicon β-subunit (red). A) An image of the entire nervous system clearly shows expression of Gal80 (green) in neurons of the abdominal ganglion, including the 14 bursicon-expressing BAG neurons (also labeled in red). The other Gal80-positive neurons in the abdominal ganglion correspond in number and position to the remaining NCCAP neurons, although we were unable to positively identify them as such due to the incompatibility of the available antibodies to CCAP. Dim labeling of two cells in the brain (arrows) is also consistent with labeling of CCAP-expressing neurons. Not obviously present are the remaining NCCAP neurons of the subesophageal and thoracic ganglia, which were labeled too weakly to be seen in this volume-rendered image. B-D) Images of single confocal sections from the same nervous system show the presence of presumptive NCCAP neurons (arrows) in the brain (B), subesophageal ganglion (C), and thoracic ganglia, T1 and T2 (D), which express Gal80 at low levels. The two grayscale panels in (C) show the Gal80 (left) and bursicon-β (right) immunoreactive signals separately for clarity. Consistent with Gal80 expression in NCCAP, the ventral, bursicon-expressing neurons of the subesophageal ganglion (BSEG) are labeled.
