The Journal of Neuroscience, January 11, 2006, ():
Single Transmembrane Domain Insulin-Like Growth Factor-II/Mannose-6-Phosphate Receptor Regulates Central Cholinergic Function by Activating a G-Protein-Sensitive, Protein Kinase C-Dependent Pathway
J. Neurosci. Hawkes et al.
26: 585
Supplemental data
Files in this Data Supplement:
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Supplementary Fig. 1: A and B, Incubation of hippocampal preparations with Leu27IGF-II (L27IGF-II), had no effect on choline acetyltransferase (ChAT) activity (A) or high-affinity choline uptake (B) at any tested concentration. C, Cyclic AMP levels in hippocampal tissues exposed to 10-8 M Leu27IGF-II (L) did not differ from those of samples incubated with high K+ Krebs buffer (K) alone, at any time point. D, Competition binding assay showing that anti-IGF-II/M6P receptor antibody, but not the pre-immune immunoglobulin can dose-dependently inhibit [125I]IGF-II binding in adult rat hippocampal membrane preparations. E, Current-voltage (I-V) relationship of mean whole-cell currents evoked by applying voltage ramps under control conditions, in the presence of pre-immune immunoglobulin alone and Leu27IGF-II (L27IGF-II, 50 nM) with pre-immune immunoglobulin (1:100). Inset shows the % decrease in whole cell currents by Leu27IGF-II alone and in the presence of the pre-immune immunoglobulin. F, I-V relationship of mean whole-cell currents evoked by applying voltage ramps under control conditions, in the presence of IGF-I receptor antagonist JB-1 (20 μg/ml) alone and Leu27IGF-II (50 nM) with JB-1. Inset shows the % decrease in whole cell currents by Leu27IGF-II alone and in the presence of JB-1.
