The Journal of Neuroscience, January 11, 2006, ():
GABAergic Signaling at Mossy Fiber Synapses in Neonatal Rat Hippocampus
J. Neurosci. Safiulina et al.
26: 597
Supplemental data
Files in this Data Supplement:
- supplemental material
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Suppl. Figure 1. Latency and rise time distributions of individual currents evoked in a CA3 pyramidal cell by minimal stimulation of granule cells in the dentate gyrus in the presence of a solution containing a Ca2+/Mg2+ ratio of 2:1.3 (A) or 1:3 (B). Note the unimodal distributions of latencies and rise times of individual responses that did not change when the extracellular Ca2+/Mg2+ ratio was changed.
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Suppl. Figure 2. L-AP4- and DNQX-sensitive synaptic responses evoked by stimulation of granule cells in the dentate gyrus. A. Left: diagram showing a CA3 pyramidal cell and a converging input from MFs. Right: unitary synaptic currents evoked in a principal cell at -70 mV by increasing stimulation intensity. Each trace is the average of 20 responses. B. The peak amplitudes of synaptic currents shown in A are plotted against different stimulus intensities. Note the all or none appearance of the synaptic currents with increasing stimulus intensity. Bars are SEM. Dashed lines connect the mean values of individual points within the same groups. C. Average responses in control and in the presence of L-AP4. Note that in this neuron synaptic currents were abolished by DNQX.
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Suppl. Figure 3. Lack of a glutamatergic NMDA-mediated component in dentate-evoked synaptic responses. A. Synaptic responses (average of 20 traces) evoked by dentate gyrus stimulation in a pyramidal cell and in a GABAergic interneuron at -70 and +40 mV in control and in the presence of L-AP4 and L-AP4 plus PTX. B. Note that the responses elicited at different voltages were symmetrical as shown by the similar values of their decay time constants (each column represents the average value obtained from 5 principal cells and 5 interneurons).
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Suppl. Figure 4. Distribution of VGLUT1 (A), ZnT3 (B), and VGAT ir the pyramidal layer (PL) and in stratum lucidum (SL) of CA3 at P5. SO: stratum oriens; Scale bar: 30 μm in A-C.
