The Journal of Neuroscience, May 17, 2006, ():
Synaptogenesis of the Calyx of Held: Rapid Onset of Function and One-to-One Morphological Innervation
J. Neurosci. Hoffpauir et al.
26: 5511
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. MNTB neurons express their glycinergic phenotype by birth. Example is from a P1 animal. The MNTB (surrounded by dashed line) is recognizable in transverse sections as a roughly oval cluster of darkly glycine-immunoreactive cell bodies. Glycine-immunoreactive neurons are also located in the LNTB and VNTB, but not prevalent in other cell groups. The boundaries of cell groups other than the MNTB are approximated from adjacent toluidine blue-labeled sections. Abbreviations: MNTB, VNTB, LNTB, medial, ventral, lateral nuclei of the trapezoid body; SPON, superior paraolivary nucleus; MSO, LSO medial, lateral superior olives; pyr, pyramidal tract; D, dorsal; L, lateral.
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Supplemental Figure 2. Ultrastructural features of the MNTB at P1 are similar to P0. Cell bodies (gold) are located very close to one another. Nuclei are eccentrically located, and nucleoli are frequently in contact with the nuclear membrane. Axons (blue) form synaptic contacts infrequently onto cell bodies. Dendrites contacted by synapses or puncta adherentia are highlighted in red if they were not linked and gold if they were linked to an MNTB cell body (such as cell 1) through our series of sections. The largest cells are numbered to indicate that they are revealed to be distinct neurons through serial sections.
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Supplemental Movie. Animated 3-D reconstruction of 4 neighboring MNTB cells. Cell bodies (transparent tan), nuclei (red), associated calyceal inputs (green, light blue, yellow, purple, dark blue, and magenta), and small inputs (orange) are color coded as shown in figures 7 and 8. Cells are referred to (clockwise beginning top right) as cells 15, 3, 18, and 17 in Results and Table 2. Three series of multiple rotations occur. The first series rotates all four cells about the vertical and then the horizontal axes. In the second series, only cells 15 and 3 are rotated three times; first in their entirety followed by two rotations with alternating cell bodies dissolved. The third series rotates cells 17 and 18 in the same manner as cells 15 and 3. Total lengths of the scale axes (end to end) seen in the first two rotations are 4 µm.
