Figure 6.
EGL-like granule neurons can be further purified to separate the outer A2B5(+) and inner TAG-1(+) EGL populations. A–C, Comparison of proliferation and developmental marker expression among A2B5(+), TAG-1(+) subpopulations and nonpanned cells (NP) by quantification of BrdU incorporation (A), Math1 expression (B), and MEF2 expression (C). Differentiation of the A2B5(+) subpopulation lags behind NP cells, as A2B5(+) cells proliferate more and show higher Math1 and lower MEF2 expression. TAG-1(+) cells represent a more differentiated subpopulation: they show almost no proliferation, no Math1 expression, and higher MEF2 expression than NP cells. BrdU, Math1, and MEF2 expression are represented as percentage of positive cells in at least three independent experiments ± SEM.