The Journal of Neuroscience, June 7, 2006, ():
Metabolism of 3-Nitrotyrosine Induces Apoptotic Death in Dopaminergic Cells
J. Neurosci. Blanchard-Fillion et al.
26: 6124
Supplemental data
Files in this Data Supplement:
- supplemental material
-
Supplemental figure 1. The incorporation of 3-nitrotyrosine into α-tubulin is reversible. Panel A: NT2 cells were either untreated (C) or treated with 3-nitrotyrosine 250 μM for 48 hours (T). After 48 hours incubation with 3-nitrotyrosine, the cells were allowed to recover in normal fresh cell media without 3-nitrotyrosine for 2, 4, 6, 8 and 24 hours. Cell lysates (40 μg) were analyzed by immuno-blotting using anti-3-nitrotyrosine, anti-Glu-tubulin, anti-α-tubulin and anti-GAPDH antibodies. Panel B: NT2 cells were treated with 3-nitrotyrosine 250 μM for 48 hours fixed and stained with polyclonal anti-3-nitrotyrosine (red) and monoclonal anti-α-tubulin (green) antibodies. Panel C: NT2 cells were treated with 3-nitrotyrosine 250 μM for 48 hours and allowed to recover for an additional 24 hours in the absence of 3-nitrotyrosine. Cells were fixed and stained with polyclonal anti-3-nitrotyrosine (red) and monoclonal anti-α-tubulin (green) antibodies. Magnification = 30x.
- supplemental material
-
Supplemental figure 2
