The Journal of Neuroscience, June 7, 2006, ():
A Role for 
1 Tubulin-Expressing Müller Glia in Regeneration of the Injured Zebrafish Retina
J. Neurosci. Fausett and Goldman
26: 6303
Supplemental data
Files in this Data Supplement:
- supplemental material
-
Supplementary Figure 1. Retinal ganglion cells from 1016α1T:GFP transgenic fish do not express GFP after optic nerve crush. Three lines of 1016α1T:GFP transgenic zebrafish (1016 L1, L2, L3) were analyzed for transgene expression in axotomized ganglion cells 4 days post crush. The transgene is not expressed in damaged axons (GFP panels) although GAP43 induction indicates ganglion cells were successfully axotomized (GAP 43 panels). The DAPI panels show merged images from each transgenic line.
- supplemental material
-
Supplementary Figure 2. 1016α1T:GFP transgene expression in the circumferential germinal zone (CGZ). In the uninjured retina, 1016α1T:GFP transgene expression is limited to cells at the CGZ (arrowhead) and newly born cells that remain GFP+ near the CGZ (arrow).
- supplemental material
-
Supplementary Figure 3. Proliferating cells migrate to other nuclear layers. Fish injured on day 0 were given a single dose of BrdU at 2dpi and sacrificed at 3, 4, 5, or 7dpi to follow the progress of the BrdU+ cell population. Labeled cells can be found in all nuclear layers at all time points, suggesting the cells had migrated soon after BrdU labeling had occurred. Some nuclei have elongated or stretched morphology, also suggesting migration (arrows). At 7dpi, numerous BrdU+ cells can be seen forming what appears to be a neurogenic cluster of proliferating cells (arrowheads), suggesting these neurogenic clusters are derived from cells in the INL that were labeled with BrdU at 2dpi. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer.
- supplemental material
-
Supplementary Figure 4. Electron micrograph of a migrating cell. Cells in the inner plexiform layer (IPL) at 5dpi are presumed to be migrating to the ganglion cell layer (GCL). (a) An example of a putative migrating cell. The inner nuclear layer (INL) is just outside the field of view to the top left, and the GCL is further to the bottom right. The identified cell appears to be a Müller cell based on the presence of lateral processes (arrows) and cytoplasm density similar to that of Müller glia (see Fig. 5f). The inset shows a higher magnification of the boxed area. A nuclear pore (white arrowhead) is visible in the nuclear envelope (black arrowheads). Note the lack of plasma membrane separating the two nuclear envelopes. (b) Pseudocolored electron micrograph of (a). The cytoplasm (green) was identified by tracing plasma membrane at high magnification. Nuclear envelopes were identified by the presence of nuclear pores (white arrowhead in (a)) and were traced to define nuclei (purple). The presence of two apparent nuclei is most likely due to sectioning through a single spiral-shaped nucleus, although it may also represent two nuclei within the same cell. The scale bar represents 2μm.
