The Journal of Neuroscience, June 14, 2006, ():
Synaptogenesis Regulates Axotomy-Induced Activation of c-Jun–Activator Protein-1 Transcription
J. Neurosci. Sung et al.
26: 6439
Supplemental data
Files in this Data Supplement:
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Figure S1. Increased AP1 expression in injured Aplysia sensory neurons does not activate apoptosis. A) At the indicated times after unilateral crush of p5-p9, neurons from the pleural ganglia on the injured and contra-lateral uninjured side were removed and examined by the MTT assay (see Methods). Cell survival is expressed as a percentage of injured neurons compared with corresponding contra-lateral uninjured neurons. Data are presented as the average from 2 separate experiments. B) TUNEL analysis for DNA fragmentation (see Methods). Five d after crushing the pedal nerves, pleural ganglia from injured and contra-lateral uninjured (CC), and from animals without nerve crush (Naive) were fixed and sectioned. Cells were labeled with fluorescein-conjugated dUTP. Some sections from naïve pleural ganglia were exposed to exogenous DNase I, as positive controls, whereas others were exposed to a reaction mixture lacking terminal deoxynucleotidyl transferase (TdT), as negative controls. C) Immunodetection of p73-c-Jun in 5 d injured and contra-lateral uninjured (CC) sensory neurons with Ab-p73-cJun. Sections were examined by confocal microscopy. Scale bar for all figures = 50 micrometers.
