The Journal of Neuroscience, June 21, 2006, ():
Glial Progenitors in Adult White Matter Are Driven to Form Malignant Gliomas by Platelet-Derived Growth Factor-Expressing Retroviruses
J. Neurosci. Assanah et al.
26: 6781
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Fig. 1
Retrovirus injection into the adult rat white matter predominantly labels NG2+/GFAP- glial progenitors. pNIT-GFP (A and B) or PDGF-IRES-GFP (C and D) was injected into the rostral white matter of adult rats (same coordinates as shown in Fig. 1). Double immunofluorescence analysis of sections near the injection tract shows that at 3 dpi with either pNIT-GFP (A-A’’) or PDGF-IRES-GFP (C-C’’) the majority of cells expressing GFP (green) also express NG2 (red). In contrast, at 3dpi with pNIT-GFP (B-B’’) or PDGF-IRES-GFP (D-D’’) the vast majority of GFP+ cells (green) do not express detectable levels of GFAP (red). All sections are stained with Hoechst nuclear dye (blue). The numbers of double positive cells (white arrows) were counted for each condition and the percentage of GFP+ cells that also express NG2 or GFAP was calculated (values on right). These values represent the mean +/- S.E.M. from multiple sections from 3 to 6 brains for each condition.
- supplemental material
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Supplemental Fig. 2
The majority of cells infected with control virus differentiate into CC1+ glia by 14 dpi. PNIT-GFP was injected into the rostral white matter of adult rats (same coordinates as shown in Fig. 1). Double immunofluorescence analysis of sections near the injection tract shows that at 14 dpi the majority of GFP+ cells have acquired a complex multipolar morphology and express CC1 (red, A-A’’) in the cell body. In contrast, the vast majority of GFP+ cells do not express detectable levels of GFAP (red, B-B’’). All sections are stained with Hoechst nuclear dye (blue). The number of double positive cells (white arrows) was counted for each condition and the percentage of GFP+ cells that also express CC1 or GFAP was calculated (values on right). These values represent the mean +/- S.E.M. from multiple sections from 3 brains.
- supplemental material
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Supplemental Fig. 3
The clonal character of a glioma in the absence (A) or presence (B) of progenitor cell recruitment. (A) shows an example of a brain tumor composed almost exclusively of a monoclonal population of tumor cells (red). Some tumor cells are infiltrating the surrounding brain tissue but there is no proliferative response of the resident glial progenitors (green). (B) shows a highly infiltrative growth pattern characteristic of gliomas. The clonal population of tumor initiating cells (red) is intermingled with a polyclonal background of proliferating glial progenitors (green). In areas the tumor-initiating cells (red) are a minority. Such tumors would still show clonal characteristics using the HUMARA assays, which can detect a clonal population even if it accounts for only 15% of total cells in a polyclonal background (Willman et al., 1994).
