The Journal of Neuroscience, June 28, 2006, ():
Layer Acquisition by Cortical GABAergic Interneurons Is Independent of Reelin Signaling
J. Neurosci. Pla et al.
26: 6924
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1. The laminar arrangement of cortical interneurons is perturbed in the absence of Reelin signaling. Coronal sections through the somatosensory cortex of P14 wild type (A-D), Dab1-/- (E-H), and reeler (I-L) mice after immunohistochemistry for Parvalbumin (A,E,I), Calbindin (B,F,J), Calretinin (C,G,K) and Nitric oxide synthase (NOS) (D,H,L). Parvalbumin+ interneurons are present in all cortical layers in wild type (A) and mutant mice (E,I), but the lamination of the immunoreactive neuropile is disrupted in the mutants. Calbindin+ cells and processes are scattered in the full width of the cortex in both Dab1 and reeler mutants (F,J), as opposed to wild type mice in which they are largely restricted to layers II-III (B). The majority of Calretinin+ interneurons occupy layers II-III in the wild type cortex (C), whereas they are also frequently found in intermediate and deep layers of the mutant cortex (arrowheads) (G,K). NOS+ interneurons are abundant in deep cortical layers and mostly absent in superficial layers in wild type mice (D), but these cells are frequently found in superficial positions in Dab1 and reeler mutants (arrowheads) (H,L). Scale bar: 300 µm.
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Supplementary Figure 2. The tangential migration of cortical interneurons is not impaired in Dab1 mutant embryos. A-D, Coronal sections through the E13.5 telencephalon of control (A and C) and Dab1 mutant (B and D) embryos showing the distribution of Lhx6-expressing cells. C,D, The abundance and distribution of migrating MGE-derived interneurons is similar in wild type (C) and Dab1 mutant (D) embryos. CP, cortical plate; H, hippocampus; LGE, lateral ganglionic eminence; lv, lateral ventricle; MGE, medial ganglionic eminence; MZ, marginal zone; NCx, neocortex; PCx, piriform cortex; SVZ, subventricular zone; VZ, ventricular zone. Scale bars: 200 µm (A,B); 50 µm (C,D).
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Supplementary Figure 3. The tangential migration of cortical interneurons is not impaired in reeler mutant mice. A-D, Coronal sections through the E13.5 telencephalon of control (A,C) and reeler mutant (B,D) embryos showing the distribution of Lhx6-expressing cells. C,D, The abundance and distribution of migrating MGE-derived interneurons is similar in wild type (C) and reeler mutant (D) embryos. CP, cortical plate; GP, globus pallidus; H, hippocampus; LGE, lateral ganglionic eminence; lv, lateral ventricle; MGE, medial ganglionic eminence; MZ, marginal zone; NCx, neocortex; PCx, piriform cortex; SVZ, subventricular zone; VZ, ventricular zone. Scale bars: 200 µm (A,B); 50 µm (C,D).
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Supplementary Figure 4. Quantification of the distribution of isochronically-transplanted cells in different regions of the cortex. Histograms of E12.5 (A,C,E) and E15.5 (B,D,F) MGE-derived cells transplanted in the motor (A,B), somatosensory (C,D) and visual (E,F) cortex of P14 mice.
