The Journal of Neuroscience, June 28, 2006, ():
Long-Term Depression at the Mossy Fiber–Deep Cerebellar Nucleus Synapse
J. Neurosci. Zhang and Linden
26: 6935
Supplemental data
Files in this Data Supplement:
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Supplemental figure 1. Anisomycin does not alter mGluR1-dependent sEPSCs.
(A) Representative sEPSCs evoked by high frequency synaptic stimulation (10 pulses at 100 Hz) with fast excitatory synaptic transmission blocked by 50 µM D-APV and 20 µM NBQX, inhibitory synaptic transmission blocked by 200 µM picrotoxin, 20 µM SR95531 and 1 µM strychnine, and glutamate transporters blocked by 50 µM DL-TBOA, with or without bath-applied 20 µ M anisomycin, from time points indicated by a (black) and b (grey) respectively on B. Traces shown are the average of 3 consecutive responses. (B) Time course of the normalized evoked sEPSC peak without (control n = 4) or with 20 µM anisomycin (n = 5). The black bar indicates the time when anisomycin was applied. (C) Time course of the normalized evoked sEPSC charge transfer in the presence and absence of anisomycin. The data from each neuron were normalized to the mean value of the first 3 responses. (D) Quantification of the % change in sEPSC peak and charge transfer averaged over the last 3 responses.
