The Journal of Neuroscience, June 28, 2006, ():
Mitochondrial Trafficking to Synapses in Cultured Primary Cortical Neurons
J. Neurosci. Chang et al.
26: 7035
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental material 1. Mitochondrial movement relative to PSD-95 clusters in a dendritic segment. The neuron was co-transfected with mito-CFP (green) and eYFP-PSD-95 (red) and time-lapse images are shown at a rate of 1 frame/20 sec. Mitochondria moving anterogradely (right to left) were seen making discrete pauses at PSD-95 clusters and elongating to bridge adjacent synaptic sites. Mitochondria shuffling between nearby synapses and dividing themselves between PSD-95 clusters were also observed.
- supplemental material
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Supplemental material 2. Distribution of mitochondrial pause times in axons and dendrites. The number of imaging frames that each mitochondrion remained in an ROI is shown for all moving mitochondria that paused during their trajectory in untreated neurons. Images were acquired at a rate of 1 frame/10 sec. Mitochondria were separated into two populations for further analysis in Fig. 6: (1) those that paused for one imaging frame, equivalent to < 20 sec and (2) those that paused for greater than one frame, equivalent to ≥ 10 sec. Data are shown from 2-3 coverslips each from 3 separate cultures
