The Journal of Neuroscience, July 5, 2006, ():
FGF Signaling Mediates Regeneration of the Differentiating Cerebellum through Repatterning of the Anterior Hindbrain and Reinitiation of Neuronal Migration
J. Neurosci. Köster and Fraser
26: 7293
Supplemental data
Files in this Data Supplement:
- supplemental material
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(Movie 1) Time-lapse movie recorded by 3D in vivo confocal microscopy showing in a lateral view the migration of neuronal precursors from the upper rhombic lip in the developing cerebellum of a gata1:GFP 781-strain embryo. GFP-expressing neuronal precursors appear around 48hpf and migrate in an anterior direction toward the MHB where they turn ventrally to settle in a neuronal cluster at the ventro-lateral edge of rhombomere 1. Migration continues for about 30 hours until it diminishes. The GFP-expressing neuronal precursors give rise to an axon fascicle that projects ventrally along the posterior border of the cerebellum and posteriorly into the hindbrain. Stacks of 13 pictures each were recorded at a distance of 3μm every 12min; pictures of individual time-points were projected in a maximum intensity projection into a single image per time-point with these images subsequently being animated as a movie at a frame rate of 6 frames per second. Abbr.: MHB: cb: cerebellar anlage, mid-hindbrain boundary, ot: optic tectum, rh: rhombencephalon, URL: upper rhombic lip.
- supplemental material
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(Movie 2) Time-lapse movie recorded by 3D in vivo confocal microscopy showing in a dorsal view the migration of neuronal precursors from the upper rhombic lip in the developing cerebellum of a gata1:GFP 781-strain embryo. GFP-expressing neuronal precursors leave the upper rhombic lip in an anterior direction toward the MHB. While cells originating from the medial third of the URL stay closer to the dorsal midline, cells from the lateral two thirds of the URL migrate along the hindbrain to populate a neuronal cluster at the ventro-lateral edge of rhombomere 1. Stacks of 21 pictures each were recorded at a distance of 3μm every 12min; pictures of individual time-points were projected in a maximum intensity projection into a single image per time-point with these images subsequently being animated as a movie at a frame rate of 6 frames per second. Abbr.: cb: cerebellar anlage, MHB: mid-hindbrain boundary, ot: optic tectum, rh: rhombencephalon, URL: upper rhombic lip.
- supplemental material
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(Movie 3) Time-lapse movie recorded by 3D in vivo confocal microscopy showing in a dorsal view the reappearance and subsequent migration of cerebellar neuronal cells from the anterior hindbrain after ablation of the entire cerebellum at 36hpf. GFP-expressing cells in the left and right lateral region of the anterior rhombencephalon emerge at 62 and 71hpf respectively (blinking white arrow). After migrating toward and along the new MHB they settle in clusters in medial and lateral positions along the MHB. From here the neuronal cells project commissural axons across the newly forming cerebellar tissue posteriorly into the rhombencephalon. Stacks of 11 pictures each were recorded at a distance of 5μm every 15min; pictures of individual time-points were projected in a maximum intensity projection into a single image per time-point with these images subsequently being animated as a movie at a frame rate of 6 frames per second. Abbr.: cb: cerebellar anlage, MHB: mid-hindbrain boundary, ot: optic tectum, rh: rhombencephalon.
