The Journal of Neuroscience, July 19, 2006, ():
Polyglutamine Proteins at the Pathogenic Threshold Display Neuron-Specific Aggregation in a Pan-Neuronal Caenorhabditis elegans Model
J. Neurosci. Brignull et al.
26: 7597
Supplemental data
Files in this Data Supplement:
- supplemental material
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Figure 1
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Figure 2
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Figure 3
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Q0 movie
QuickTime movies of motility in young adult animals expressing polyQ proteins. To demonstrate the range of motility in these lines, we show 10 second movies of ~10, four day old animals in M9 buffer expressing extrachromosomal arrays of Q86::YFP or Q0::YFP. The movies show that Q0::YFP animals display rapid, sinusoidal body bends in regular rhythm. In contrast, Q86::YFP animals move less frequently and in a more erratic pattern. Heterogeneity of thrashing behavior can be seen in Q86 animals. For example one animal (far top left) moves rapidly at the start of the movie but later slows and becomes characteristically erratic. Data from motility assays (Fig. 4 and Fig. S3) use the average thrashing rate quantified over 1 minute, rather than 10 seconds shown in these movies. Quantification of these results reveals that bursts of sinusoidal movement in Q86 animals are infrequent compared to normal movement. Motility is assayed by examining thrashing rates of a single animal, rather than a population as shown here.
- supplemental material
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Q86 movie
