The Journal of Neuroscience, January 18, 2006, ():
Requirement of Adenylate Cyclase 1 for the Ephrin-A5-Dependent Retraction of Exuberant Retinal Axons
J. Neurosci. Nicol et al.
26: 862
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure: Quantification of labeled retinal axons.
The complete sequence that was used to quantify the surface occupied by the labeled retinal axons is illustrated in one case of a temporal eGFP-retinal explant.
(A): The original reconstruction after the acquisition of 30 video frames (x10 objective, Zeiss epifluorescence microscope) that have been aligned with Metamorph.
(B): Setting the inclusive threshold.
(C): Overlay of the rectangular grid that separates the dorsal (D1) to ventral (D5) bins, and the rostral (R’1) to caudal (R’20) bins.
(D): Overlay of a second type of grid that is drawn to fit the curved contours of the SC (R1 … R20)
(E): Histogram of the distribution of labeled axons along the dorso-ventral axis for this culture.
(F): Curve of the distribution of labeled axons along the rostro-caudal axis (R1 to R20 grid) for this culture).
- supplemental material
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Video: AC1brl/brl retinal axons do not retract after an ephrin-A5-induced collapse.
Films of WT (left panel) and AC1brl/brl (right panel) growth cones collapses induced by ephrin. The growth cones were filmed 1 hour before and 1 hour and a half after ephrin-A5 application, to the medium. This event indicated by a dark circle appearing in the upper left part of the figure. The film includes one image per min. In both cases the axons stopped growing and the lamellipodia disappeared rapidly from the growth cone following the ephrin-A5 application. After which the WT axon retracted and forms a long trailing process, whereas the AC1brl/brl retinal axon remains in the same position 1 hour and a half after the ephrin-A5 application.
