The Journal of Neuroscience, July 26, 2006, ():
Tenascin-R Restricts Posttraumatic Remodeling of Motoneuron Innervation and Functional Recovery after Spinal Cord Injury in Adult Mice
J. Neurosci. Apostolova et al.
26: 7849
Supplemental data
Files in this Data Supplement:
- supplemental material
-
Supplemental figure 1. (A) Individual values of the product foot-stepping angle (FSA) x rump-height index (RHI) plotted against the FSA values of “moderately” and “severely” injured C57BL/6J mice at 6 weeks after surgery. There is a good linear correlation if two “outlier” values (black squares, “severely” injured mice) are excluded. One of the mice with “outlier” FSA x RHI values is shown in a frame from a video recording of a beam walking trial. For further explanations see the main text.
- supplemental material
-
Supplemental figure 2. Frequency distribution diagrams of motoneuron soma areas (A) and densities of ChAT+ (B), VGAT+ (C) and VGLUT1+ (D) perisomatic puncta in samples from intact (Int) and spinal cord-injured (SCI) TNR-deficient mice (TNR-/-) and wild-type (TNR+/+) littermates. Number of animals studied per group are indicated in (A). Pairs of arrows indicate significantly different distributions (p < 0.05, two-sample Kolmogorov-Smirnov test). Also significantly different in the same test were the distributions for both injured groups compared to the intact groups in panels (A-B) and between injured TNR-/- and intact TNR-/- mice in panel D.
- supplemental material
-
Supplemental figure 3. Perineuronal nets around motoneurons in intact and lesioned spinal cords. (A,B) Single confocal optical slices (1.5 µm-thick) from Wisteria floribunda agglutinin (WFA) stained parasagittal sections from the lumbar spinal cord of a non-lesioned TNR+/+ mouse (A) and a non-lesioned TNR-/- (B) mouse. Note the coarser, grainy appearance of the staining around the cell body and the less clear delineation of the proximal dendrite in the TNR-/- mouse (B) compared to those in the TNR+/+ littermate (A, dendrites at arrows in both panels). (C,D) Merged stacks of confocal slices showing WFA stained cells within less than 500 µm distal to the lesion scar in the spinal cord of a TNR+/+ (C) and a TNR-/- (D) mouse studied one week after injury. (E,F) Confocal stack images showing intense TNR immunofluorescence labeling of a perineuronal net (arrow) and the surrounding neuropil in a wild-type mouse (E) and background signal in a TNR-/- mouse (F). Both animals were studied one week after lesion and the panels show areas close to the lesion scar. Scale bar in B indicates 50 µm for all panels.
