The Journal of Neuroscience, July 26, 2006, ():
Origin of Oligodendrocytes in the Subventricular Zone of the Adult Brain
J. Neurosci. Menn et al.
26: 7907
Supplemental data
Files in this Data Supplement:
- supplemental material
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Suppl data 1. Olig2 is expressed in the adult mouse SVZ
(A) Coronal section of adult CD-1 mouse tissue immunostained with an anti-Olig2 antibody (red) and counterstained with the green nuclear marker cyquant. Arrows indicate Olig2+ cells located in the SVZ. (B) Adult CD1 mice received a single intraperitoneal injection of BrdU (50 g/kg of body weight) 1 hr before their sacrifice. Coronal brain sections immunostained with BrdU (red) and Olig2 (green). Insert shows a higher magnification of an adjacent tissue section. 21.9% of Olig2+ cells located in the SVZ were also BrdU+ (arrows). Arrowheads in inset indicate Olig2- / BrdU+ SVZ cells presenting an elongated morphology. (C) Plastic 1.5 μm thick section stained for Olig2 (brown DAB deposit) and counterstained with toluidine blue. Over 93 Olig2+ SVZ cells studied in 1.5 μm thick sections, 11 were in mitosis (arrow), confirming that Olig2+ SVZ cells are actively proliferating.
CC: corpus callosum; LV: lateral ventricle; Str. : striatum ; SVZ: subventricular zone.
Scale bar: A, B : 90 μm ; inset in B: 35 μm; C: 10 μm.
- supplemental material
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Suppl data 2 . Time-course of the cell types produced after RCAS-AP infection.
The time course of appearance of new oligodendrocytes in the adult brain SVZ was studied by injecting RCAS-AP retrovirus into the SVZ of P60-120 Gtv-a mice. The morphology and location of AP+ cells was analyzed in the SVZ, CC and RMS/OB at 1.5 (n=5), 4 (n=5), 10 (n=5), 15 (n=4), 21 (n=22), and 240 (n=10) days after injection. This table indicates the number (average +/- SEM) and the morphology of AP+ cells found at different survival times after RCAS-AP infection. Representative camera lucida drawings of the cell morphologies observed are shown. AP+ cells were counted in 1 every 4 50μm-thick horizontal serial section through the whole brain. Note that both young oligodendrocytes and neuroblasts were detected as soon as 4 days after retroviral infection. Similarly, both mature myelinating oligodendrocytes and differentiated neurons start to appear 10 days after infection. Interestingly, AP+ mature oligodendrocytes were still present four months after the initial injection, suggesting that these cells became incorporated into the adult mouse brain.
nd: not determined
