The Journal of Neuroscience, July 26, 2006, ():
Probing the Regulation of M (Kv7) Potassium Channels in Intact Neurons with Membrane-Targeted Peptides
J. Neurosci. Robbins et al.
26: 7950
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary video
The video shows the perfusion of a fluorescent palpeptide over three rat ganglion cells and its subsequent retention in the cell membranes after washout. Initially the sensitivity of the camera is set lower than background autofluorescence. At “peptide on”, 10 µM of the fluorescein-tagged (Lys-8) PIP2-palpeptide was added to the bathing solution for 1 minute. Fluorescence appears in the solution and becomes concentrated in the cell membranes. When “peptide on” disappears from the screen, the bathing medium was replaced with peptide-free solution. The fluorescence in the bathing solution is then washed out (after 2 minutes) but is retained in the plasma membrane of the cells. Video speed is 15X faster than real time.
