The Journal of Neuroscience, August 9, 2006, ():
Compensation of Inositol 1,4,5-Trisphosphate Receptor Function by Altering Sarco-Endoplasmic Reticulum Calcium ATPase Activity in the Drosophila Flight Circuit
J. Neurosci. Banerjee et al.
26: 8278
Supplemental data
Files in this Data Supplement:
- supplemental material
-
Movie 1: Real time video recording of air-puff induced flight in itprka1091/ug3 and Kum170/+; itprka1091/ug3 animals.
Flies were prepared for recording as described in experimental procedures for electrophysiological recording. Following a gentle air-puff, Kum170/+;itprka1091/ug3 (two flies in right bottom corner) animals exhibit brief periods of wing beating, while itprka1091/ug3 flies are unable to initiate wing beating (two flies in left up corner).
- supplemental material
-
Figure 1. Two classes of calcium signals shape the Drosophila flight response to an air-puff stimulus.
Normal flight behavior requires accurate assembly of the cognate neural circuit. Two kinds of InsP3 mediated Ca2+ signals function in this process. One class can be manipulated by altering levels of Gq and PLCβ as well as Ca2+i by altering SERCA activity (left panel). Based on our genetic analysis, we speculate that this class of signals comprise of a slow rise in Ca2+. On the contrary, Ca2+ signals required for maintenance of flight patterns depend predominantly upon the intrinsic properties of the InsP3R (thick arrow) which can generate fast oscillatory Ca2+ signals. Though Gq, PLCβ and SERCA continue to act, they have a minimal influence on this class of signals (panel on the right).
