The Journal of Neuroscience, August 9, 2006, ():
Calcium Activation of the LMO4 Transcription Complex and Its Role in the Patterning of Thalamocortical Connections
J. Neurosci. Kashani et al.
26: 8398
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure1: Thalamic axons innervating barrel cortex fail to segregate in LMO4 mutant mice. DiI crystals were injected into the thalamus of paraformaldehyde-fixed control (A and B) and conditional lmo4 null (C and D) littermates at P15. After 4 weeks, coronal sections were counter stained with Hoechst and the organization of thalamocorical afferents was visualized by confocal fluorescence microscopy. Thalamocortical afferents were clearly organized into barrels in control mice (A and B), but the segregation of axons was disrupted in lmo4 mutant mice (C and D).
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Supplemental Figure 2: Cellular organization of layer 4 of barrel cortex is disrupted in LMO4 mutant mice. Coronal (A and B) or tangential sections (C and D) from P17 conditional null (n=3) and control mice (n=2) were stained with Hoechst for visualization of barrel septa. Knockout mice (B and D) show no discernable barrel septa whereas control littermates have light and dark patches corresponding to barrel septa and hollows respectively.
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Supplemental Figure 3: Dendritic development of callosally-projecting neurons is grossly normal in LMO4 mutant mice. Layer 2/3 neurons in wild-type (A-C) and conditional lmo4 null mice(D-F) retrogradely labeled with DiI from the contralateral cortex at P11. The dendritic morphologies of neurons in the two cases are not substantially different.
