The Journal of Neuroscience, August 23, 2006, ():
A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate-Induced Responses in Drosophila
J. Neurosci. Ha and Smith
26: 8727
Supplemental data
Files in this Data Supplement:
- supplemental material -
Supplemental Table 1. PCR primers were used to assay expression of odorant and gustatory receptors expressed in the Drosophila antenna. Each primer pair for each receptors gene spanned an intron to distinguish cDNA products from genomic DNA contamination.
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Supplemental Figure 1. Odor responses of olfactory neurons in large basiconic sensilla among control flies, tod1, and tot1 mutants. To classify each large basiconic sensillum type in third antenna segments (de Bruyne et al., 2001), we used a set of 12 different odor stimuli including VA and the negative control odors, air and paraffin oil. The bar graphs show each response measured as the increase in spikes per second compared to the spontaneous firing rate prior odor stimulation. All odorants were diluted 10-2 in paraffin oil except for 2,3-butanedione that was diluted 10-2 in water. To avoid cross contamination between odorants, each odorant was freshly prepared prior to use for these experiments. These graphs were based on recordings from at least 5 different sensilla. Each data point represents the mean ± S.E.M.
