The Journal of Neuroscience, September 6, 2006, ():
Unipolar Brush Cells of the Cerebellum Are Produced in the Rhombic Lip and Migrate through Developing White Matter
J. Neurosci. Englund et al.
26: 9184
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1 Migration pathways of Tbr2+ UBCs in cerebellar hemispheres (E19.5).
A-D, Sections from lateral (A) to medial (D) through the same hemisphere, showing Tbr2 immunofluorescence (red) and DAPI counterstain (blue). Numbers indicate distance (in µm) from the lateral edge of the hemisphere. At all levels, Tbr2+ cells appeared to follow two main pathways from the rhombic lip (rl): a rostral pathway towards brainstem (lower arrows in A, D), and a dorsal pathway towards cerebellar cortex (upper arrows in A, D). The rostral pathway appeared to predominate in lateral sections (A, B), and the dorsal pathway in medial sections (C, D). Other abbreviations as in previous figures. Scale bar: 200 µm.
- supplemental material
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Supplementary Figure 2 Migration of rhombic lip (rl) cells into developing white matter at all mediolateral levels in organotypic slice co–culture.
Tbr2 (red) and GFP (green) immunofluorescence are shown with DAPI counterstain (blue) in a cryosection through co–cultured coronal slices of E17.5 tau–GFP transgenic rhombic lip and non–transgenic cerebellum after 4 DIV. In such coronal slices, typically only one tissue slice per brain contained rhombic lip. This view of one half of an entire coronal slice (midline at right) shows that Tbr2+/tau–GFP+ rhombic lip cells migrated into the developing white matter at all mediolateral levels (arrowheads) and avoided the deep cerebellar nuclei (dcn). Other abbreviations as in previous figures. Dashed white line indicates the approximate boundary between tau–GFP transgenic rhombic lip and non–transgenic cerebellar slice. Orientation: dorsal (D) top, lateral (L) left. Other abbreviations as in previous figures. Inset: diagram of E17.5 sagittal section showing approximate plane of coronal sections in this experiment (red dashed lines). Scale bar: 100 µm.
