The Journal of Neuroscience, September 20, 2006, ():
Identification of Sox17 as a Transcription Factor That Regulates Oligodendrocyte Development
J. Neurosci. Sohn et al.
26: 9722
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure S1. Western blotting and immunohistochemical characterization of Sox17 antiserum. (A) Western blot analysis of COS7 cells transiently transfected with pCMV vector (-, control lane), CMV-driven Sox17 or Sox10 expression plasmids to demonstrate antibody specificity. Sox17**: Western blot incubated with the anti-recombinant Sox17 antisera primarily used in this study (Kanai et al., 1996). Sox17*: same samples analyzed with anti-human Sox17 antibody (RnD Systems). Both Sox17 antibodies detected bands of identical molecular weight. Sox10 was detected with Abcam Sox10 antibody. (B and C) Sox17 immunolabeling in white matter of P5 mouse ventral spinal cord. Coronal sections of mouse spinal cord stained with SOX17 antibodies from Kanai et al., 1996 (B) and from RnD systems (C). These two distinct antibodies give the same labelling pattern. Dotted line delineates the ventral edge of the section. Scale bar= 50 μm.
- supplemental material
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Supplemental Figure S2. Immunocytochemical characterization of FACS-purified CNP-EGFP+ cells at different developmental stages. CNP-EGFP+ cells were immunostained after FACS purification at postnatal days 4, (P4), 15 (P15) and 30 (P30). Cells were plated for 1-2 hr before immunostaining with NG2 (blue), O4 (red), or O1 (yellow) antibodies. Data are averages from one representative sorting experiment that was repeated twice. Cells from a total of 6-12 brains were pooled. Data are averages + S.E.M. The total number of cells counted for each antibody at each age ranged between 450 and 1150 (20-35 microscopic fields).
