The Journal of Neuroscience, September 27, 2006, ():
Na+, Cl–, and pH Dependence of the Human Choline Transporter (hCHT) in Xenopus Oocytes: The Proton Inactivation Hypothesis of hCHT in Synaptic Vesicles
J. Neurosci. Iwamoto et al.
26: 9851
Supplemental data
Files in this Data Supplement:
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Supplemental Figure. I(V) curves determined in frog oocytes transfected with hCHT LV mutant in 10 μM choline at different pH. The choline-induced currents decline at low pH at fixed membrane voltage, making reversal potential values prone to error. The reversal potential occurs in a narrow range of 30-50 mV, which is near the calculated Na+ reversal potential (38.6 mV). These data indicate that the primary current carrier is likely to be Na+, but it does not exclude other ions, in particular protons, from permeating hCHT.
