The Journal of Neuroscience, September 27, 2006, ():
Targeted Deletion of a Single Sca8 Ataxia Locus Allele in Mice Causes Abnormal Gait, Progressive Loss of Motor Coordination, and Purkinje Cell Dendritic Deficits
J. Neurosci. He et al.
26: 9975
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1. CRE recombinase is specifically and efficiently expressed only in cerebellar Purkinje cells in Pcp2-Cre transgenic mice.
(a) Structure of the Pcp2-Cre transgenic allele. The 835 bp Purkinje cell-specific (Pcp-2) promoter was used to regulate the 1.3 kb of Cre gene, and a 240 bp SV40 polyadenylation signal was added. We established three transgenic lines from this construct and used founder P-CRE-3 mouse line for further study.
(b) P-CRE-3 mice were crossed with R26R mice and the P-CRE-3/R26R mice to demonstrate selectively expression of recombinase Cre in cerebellar Purkinje cells. X-gal staining of sagittal cerebellum sections of 4-week-old, 8-week-old and 12-week-old P-CRE-3/R26R compound heterozygous mice all demonstrated cerebellar Purkinje cell-specific lacZ expression activity following the specific Cre recombination. Arrows in those sections indicate Purkinje cells. Whole-brain staining from 14-week-old P-CRE-3/R26R mice shows a cerebellum-specific (Black arrow) β-gal activity while the brain from 14-week-old R26R heterozygous mouse was negatively stained. Note that β-gal positive Purkinje cells appear less frequent at the age of 4-week-old and the frequency and density of stained Purkinje cells steadily increased from 4 weeks to 12 weeks.
