The Journal of Neuroscience, January 25, 2006, ():
Splice Variants of the NR1 Subunit Differentially Induce NMDA Receptor-Dependent Gene Expression
J. Neurosci. Bradley et al.
26: 1065
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1. Ca2+ homeostasis is not adversely affected in NR1–/– neurons and in NR1–/– neurons transfected with NR1-1a. a, Measurement of peak [Ca2+]i responses revealed a selective loss of NMDA-induced [Ca2+]i increase in NR1–/– neurons. b, Basal [Ca2+]i was slightly but significantly lower in NR1–/– neurons than in NR1+/+ and NR1+/– neurons; each condition represents the mean ± SEM for at least 75 neurons from at least four embryos. Basal [Ca2+]i was calculated as the average of six baseline ratio values before stimulation with NMDA or K+. c, K+-induced [Ca2+]i responses were not significantly different in NR1+/– neurons and NR1–/– neurons transfected with NR1-1a; each condition represents the mean ± SEM for at least 18 neurons from at least four embryos. *P < 0.05; unpaired t-test. A.U.; arbitrary units.
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Supplementary Figure 2. Mutant CaM reduces gene expression with different stimuli. Transfection of dominant-interfering CaM, CaM1234, into NR1+/– neurons significantly reduced CRE-dependent gene expression induced by NMDA (30 μM, a), K+ (55 mM, b), or forskolin (10 μM, c), and SRE-dependent gene expression induced by BDNF (20 ng/ml, d). Basal gene expression is reduced by CaM1234 in each case. Also in each case, fold induction (Stim/Basal) is unchanged by CaM1234. Means ± SEM for at least three cell preparations. * P < 0.01; unpaired t-test. A.U.; arbitrary units.
