The Journal of Neuroscience, January 25, 2006, ():
Dendrites Contain a Spacing Pattern
J. Neurosci. Taylor and Fallon
26: 1154
Supplemental data
Files in this Data Supplement:
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Supplemental Methods
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SupFig 1. PADRENs in two 17 DIV hippocampal neurons. (A,D) Immunostaining before application of detergent using the mouse 5F9 anti-MAP2 antibody (B,E) Immunostaining after application of detergent using rabbit polyclonal anti-MAP2 antibodies, (C,F) merge. Scale bar 30 um.
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SupFig 2. Montage of PADRENs in 17 DIV hippocampal neurons. (A) Immunostaining before application of detergent using the mouse 5F9 anti-MAP2 antibody, (B) merge. Scale bar 30 um.
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SupFig 3. PADRENs in 10 DIV (A-B) cortical and (C-D) cerebellar neurons. (A,C) Immunostaining before application of detergent using the mouse 5F9 anti-MAP2 antibody, (B,D) merge. Scale bar 30 um.
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SupFig 4. (A) A histogram showing the nearly normal distribution of wavelengths in 17 DIV hippocampal neurons (p = 0.02 ; Kolmogorov-Smirnov test; data versus a normal distribution with the same mean and standard deviation). An exponential probability density function with the same mean is illustrated for comparison and is significantly different (p < 1 × 10-10; Kolmogorov-Smirnov test; data versus an expontial distribution with the same mean). (B) A line graph showing the change over time in percent of PADRENs containing a branch, percent of dendrite each PADREN-type occupies, and branch density on PADRENs relative to 8 DIV values. Absolute values at 8 DIV are shown in table at bottom (PADRENs contain exactly one branch point in > 95% of cases). The percent of PADREN1s containing at least one branch decreases over time (8 vs. 17 DIV: p = 0.037) as does the percent of dendrite that they occupy (8 vs. 17 DIV: p = 0.001). A converse trend occurs with PADREN2s. Branch density on PADREN1s and PADREN2s does not change significantly over time (Kruskal-Wallis).
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SupFig 5. Signal in each gray-scale image is a cocktail of phalloidin and rabbit anti-synapsin 1, which labels the F-actin cytoskeleton and pre-synaptic terminals respectively. Color images are a merge of the immunostaining resulting from the mouse 5F9 anti-MAP2 antibody before application of detergent and the corresponding gray-scale image shown. (A-D) Representative 8 DIV hippocampal dendrites. (E-H) Representative 17 DIV hippocampal dendrites. Scale bar 15 um.
