The Journal of Neuroscience, January 25, 2006, ():
Transition from Reversible to Persistent Binding of CaMKII to Postsynaptic Sites and NR2B
J. Neurosci. Bayer et al.
26: 1164
Supplemental data
Files in this Data Supplement:
- supplemental material
-
Supplemental Fig 1: Semi-quantitative assessment of post-synaptic translocation of GFP-CaMKIIα, plotted as a cumulative percentage (n=3 experiments; 50-80 neurons per experiment). Transfected neurons (12-14 DIV) incubated overnight with or without AP-5 were fixed and immunostained for PSD95; transfected neurons stimulated with glutamate/glycine (100/10μM) for 1 min were previously incubated with AP-5 overnight; immediately after stimulation they were fixed and immunostained for PSD95. An observer, blind to the condition, evaluated the fluorescence contrast between synaptic areas (PSD95 positive) and the rest of the dendrites on a scale of “–“ to “++++”. Representative examples of the scoring applied are shown on the right. Scale bar: 5 μm.
- supplemental material
-
Supplemental Fig 2: Effect of S- and T-site mutations on the transient and persistent post-synaptic translocation of CaMKIIα, following a 1 min glutamate/glycine stimulus. A, Time lapse images of GFP-CaMKIIα in dendrites of neurons pre-incubated with AP-5 (100 μM) for 16 h. Arrowheads point to examples of synapses where GFP-CaMKIIα accumulated or not (see F98K) during the stimulation. Quantitative analyses of these time series are shown in Fig 9. Scale bar: 10 μm.
- supplemental material
-
Supplemental movie: Hippocampal neuron (12-14 DIV) transfected with GFP-CaMKIIα was stimulated with glutamate/glycine for 1 min and washed for 27 min in low Ca2+/high Mg2+ external solution, as indicated. Frames were collected every 10 s (time shown in lower right corner) during the first 4 min and then every 30 s and are displayed at 6 frames per sec.
