The Journal of Neuroscience, January 25, 2006, ():
Synaptic Vesicle Protein 2 Enhances Release Probability at Quiescent Synapses
J. Neurosci. Custer et al.
26: 1303
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure S1. Absolute EPSC amplitudes are reduced at all series resistance values in SV2A and SV2A/B knockout neurons relative to littermate controls
Autaptic EPSCs were recorded from hippocampal neurons in the whole-cell voltage clamp configuration. Amplitudes are the average of three responses separated by at least 10 seconds. Series resistance was compensated up to 70% and the values reported are post-compensation.
(A, B) EPSC amplitudes as a function of series resistance for (A) wild type and SV2A knockout neurons and (B) SV2B and SV2A/B knockout neurons. Best fit lines indicate that EPSC amplitudes are reduced at all series resistance values in SV2A knockout and SV2A/B double knockout neurons relative to littermate controls. Therefore the reduced mean EPSC amplitudes observed in SV2A and SV2A/B knockouts did not reflect differences in our ability to adequately voltage clamp cells. Data are from littermate cultures of 6 wt and SV2A KO animals, and 23 SV2B KO and double KO animals.
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Supplemental Figure S2. Amplitude and charge distributions of miniature EPSCs recorded from wild type and SV2 knockout neurons.
Miniature EPSCs were recorded from hippocampal neurons on microislands. Data represent 1112 events from 14 wild type neurons, 519 events from 10 SV2A knockouts, 777 events from 9 SV2B knockouts, and 1501 events from 14 SV2A/B knockout neurons.
(left panels) mEPSC amplitude distributions for wild type, SV2A knockout, SV2B knockout and SV2A/B double knockout neurons.
(right panels) mEPSC charge distributions for wild type, SV2A knockout, SV2B knockout and SV2A/B double knockout neurons.
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Supplemental Figure S3. Synaptic depression is reduced in SV2A knockouts
Neurons from wild type, SV2A knockout, SV2B knockout, and SV2A/B double knockout cultures were stimulated at a frequency of 10 Hz. Amplitudes are normalized to the initial response. Data are mean ± SEM. Numbers of cells analyzed are indicated in parentheses. Data are from 2 wt, 2 SV2A KO, 6 SV2B KO, and 6 double KO cultures. In response to 10 Hz trains SV2A knockout neurons exhibited reduced synaptic depression relative to WT and SV2B knockout neurons, but their phenotype is not as severe as that of SV2A/B double knockouts.
