The Journal of Neuroscience, October 18, 2006, ():
Formation of Presynaptic Terminals at Predefined Sites along Axons
J. Neurosci. Sabo et al.
26: 10813
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1. Histogram of pause probability in 10 DIV neurons. The pause probability distribution changed with neuronal development from a Gaussian distribution seen at 4 DIV (see Fig. 2) to the more binary distribution shown here (n = 47 vesicles).
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Supplementary Figure 2. Intracellular calcium regulates STV pausing. Plots of the mean (+/- SE) of pause probability, pause frequency and pause duration without (open bars) and with (grey bars) BAPTA loading. All data were normalized to mean values derived from the control condition. *, p < 0.05.
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Supplementary Figure 3. Latrunculin A treatment disrupts the neuronal actin network. Neurons were labeled with phalloidin (red), which binds to actin filaments, and antibodies to β III tubulin (green) either in the absence (A) or presence (B) of latrunculin A treatment. Imaging parameters were identical for the two conditions. The top panels of A and B show neuronal cell bodies and proximal axons, while the bottom panels show higher magnification views of more distal axons. Scale bars, 10 μm.
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Supplementary Movie. Movie demonstrating that dendritic filopodia form stable contacts at pause sites. STVs were labeled with FM4-64 (red), and the fluorescence images were superimposed on the transmitted light images to allow simultaneous identification of pause sites and contact sites. Prior to contact, STVs paused at the site indicated (red arrow). The approaching dendritic growth cone extended a filopodium (green arrow) that eventually stabilized at the pause site. Times for each frame are indicated in black.
