The Journal of Neuroscience, October 18, 2006, ():
Role of Bone Morphogenic Protein 2 in Retinal Patterning and Retinotectal Projection
J. Neurosci. Sakuta et al.
26: 10868
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. Quantification of the effect of BMP2 knockdown on the expression of topographic molecules along the D-V axis. Signal intensities in in situ hybridization at the positions 1-4 at regular intervals along the D-V axis of the retina (J) were measured using NIH Image J. Signal intensities were expressed as the percentage compared with the signals where the expression of each molecule is absent (0%) and the most abundant (100%). Error bars indicate standard deviation (n = 5-9). Quantification of the expression of Tbx2 (A), Tbx3 (B), Tbx5 (C), ephrin-B1 (D), ephrin-B2 (E), Ventroptin (F), cVax (G), EphB2 (H), and EphB3 (I) in the E8 retina. (J) Schematic representation of the retina showing the positions used for quantification of in situ hybridization signal.
- supplemental material
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Supplemental Figure 2. Quantification of the effect of BMP2 knockdown on the expression of topographic molecules along the N-T axis. Signal intensities in in situ hybridization at the positions 1-4 at regular intervals along the N-T axis of the retina (Supplemental Fig. 1J) were measured (n = 4-7). Quantification of the expression of FoxG1 (A), FoxD1 (B), SOHo1 (C), GH6 (D), ephrin-A2 (E), ephrin-A5 (F), and EphA3 (G) in the E8 retina.
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Supplemental Figure 3. Quantification of the effect of BMP2 misexpression on the expression of topographic molecules along the D-V axis. Signal intensities in in situ hybridization at the positions 1-4 at regular intervals along the D-V axis of the retina (Supplemental Fig. 1J) were measured (n = 5-9). Quantification of the expression of Tbx2 (A), Tbx3 (B), Tbx5 (C), ephrin-B1 (D), ephrin-B2 (E), Ventroptin (F), cVax (G), EphB2 (H), and EphB3 (I) in the E8 retina.
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Supplemental Figure 4. Quantification of the effect of BMP2 misexpression on the expression of topographic molecules along the N-T axis. Signal intensities in in situ hybridization at the positions 1-4 at regular intervals along the N-T axis of the retina (Supplemental Fig. 1J) were measured (n = 3-7). Quantification of the expression of FoxG1 (A), FoxD1 (B), SOHo1 (C), GH6 (D), ephrin-A2 (E), ephrin-A5 (F), and EphA3 (G) in the E8 retina.
- supplemental material
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Supplemental Figure 5. Flat-mount in situ hybridization of the retina. Ephrin-B1 is expressed in a dorsal-high pattern at E5 (A) and in a D/T-high pattern at E8 (B). EphB2 is expressed in a ventral-high pattern at E5 (C) and in a V/N-high pattern at E8 (D). Remnants of the pigment epithelium still attached to the periphery of the neural retina (A-C). Nasal and dorsal are left and top, respectively.
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Supplemental Figure 6. Quantification of the retinotectal projection in BMP2 knockdown and ephrin-A2-misexpressed embryos. The tectum was divided into 10 segments along the A-P and M-L axes, and numbers of the terminals of labeled dorsotemporal axons per each segment of each tectum were counted. The distributions of the axon terminals in the control (circle; n = 5), BMP2 knockdown (triangle; n = 5), and ephrin-A2-misexpressed (square; n = 8) embryos along the A-P (A) or M-L (B) axis are shown. Error bars indicate standard deviation. Numbers 1-10 represent 10 different positions along the A-P or M-L axis.
