The Journal of Neuroscience, October 25, 2006, ():
Survival Motor Neuron Function in Motor Axons Is Independent of Functions Required for Small Nuclear Ribonucleoprotein Biogenesis
J. Neurosci. Carrel et al.
26: 11014
Supplemental data
Files in this Data Supplement:
- supplemental material -
Supplementary Figure 1: Statistical comparisons of MO and RNA injections. Beyond those reported in this text, multiple statistical comparisons were conducted to thoroughly analyze the results obtained from the injections described. While the results of the non-parametric Mann-Whitney tests were indicated, Chi square analysis of the distribution was also performed. In all incidences, the resulting p-values of both tests agreed. HA, HA-tagged protein. All other abbreviations as in Figure 3 and text.
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Supplementary Figure 4. Statistical comparisons of Kaplan-Meier data. Beyond those reported in this text, multiple statistical comparisons were conducted to thoroughly analyze the results obtained from the survival curves using the log-rank values from the pair wise comparisons of the longevity of smn morphant fish (A) and those co-injected with smn MO and RNA (B). n, the number of fish evaluated in each experimental group.
- supplemental material
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Supplementary Figure 2. The Q282A mutation has a dominant effect. Injection of the Q282A RNA alone can cause motor axon defects (n=213 fish, 4260 nerves) that are statistically different from injections of the control MO and the hSMN RNA alone. (p<0.001 versus both the control MO and hSMN).
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Supplementary Figure 3. Forms of SMN incapable of rescuing motor axons defects are stable. (A) Western blot analysis of smn MO injected embryos co-injected with either full-length HA-tagged SMN (lane 1), HA-tagged SMNΔ7 (lane 2), HA-tagged SMN read-though (lane 3), HA-tagged SMN A111G (lane 4), or HA-tagged SMN Q282A (lane 5). After injection with smn MO and respective construct, embryos were harvested for protein at 26-28 hpf. (B) Western blot analysis of smn MO injected embryos co-injected with full-length HA-tagged SMN and protein analyzed at 1 dpf (lane 1), 3 dpf (lane 2), and 6 dpf (lane 3). β-actin is shown as a protein loading control.
