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The Journal of Neuroscience, November 8, 2006, ():

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Release of Full-Length EphB2 Receptors from Hippocampal Neurons to Cocultured Glial Cells
J. Neurosci. Lauterbach and Klein 26: 11575

Supplemental Data

Files in this Data Supplement:

• supplemental material - Supplemental material
• supplemental material - Supplementary Figure 1: GFAP-positive cells in dissociated 14 DIV hippocampal cultures. 14 DIV primary hippocampal low-density cultures were fixed, permeabilized and immunostained with antibodies against the glial fibrillary acidic protein (GFAP) to reveal the morphology of astrocytes in hippocampal cultures (GFAP, right column, A’-B’). The corresponding bright-field images are shown on the left (phase, A-B). Scale bars = 10 µm.
• supplemental material - Supplementary Figure 2: Expression of ephrinBs and EphB receptors in 14 DIV hippocampal cultures. 14 DIV primary hippocampal low-density cultures were stimulated for 15 min with preclustered unfused Fc protein (negative control), ephrinB2-Fc, EphB1-Fc or EphB4-Fc, fixed and stained as described in Figure 1. Scale bars = 10 µm.
• supplemental material - Supplementary Figure 3: Expression of ephrinAs and EphA receptors in 14 DIV hippocampal cultures. 14 DIV primary hippocampal low-density cultures were stimulated for 15 min with preclustered unfused Fc protein (negative control), or the indicated ephrinA-Fc, and EphA-Fc fusion proteins, fixed and stained as described in Figure 1. Scale bars = 10 µm.
• supplemental material - Supplementary Figure 4: Comparison of endogenous and exogenous EphB2 expression. 14 DIV hippocampal high-density cultures were transfected with expression constructs encoding either EphB2-YFP (A-D, I-L) or YFP (E-H). Two days later, cultures were stimulated with either preclustered ephrinB1-Fc (A-H) or as a control with preclustered Fc (I-L) for 15 min, fixed and stained as described in Figure 1. Left row shows merge of YFP fluorescence of transfected neurites in red and the ephrinB1-Fc immunostaining in green. YFP fluorescence depicts exogenous EphB2 expression (C), whereas ephrinB2-Fc stains both endogenous EphBs and exogenous EphB2 (A,B). Right row shows phase contrast. Scale bars = 10 µm.
• supplemental material - Supplemental movie 1
• supplemental material - Supplemental movie 2
• supplemental material - Supplemental movie 3
• supplemental material - Supplemental movie 4
• supplemental material - Supplemental movie 5

This Article Abstract Full Text Submit an eLetter Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via Web of Science (8)

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