The Journal of Neuroscience, November 8, 2006, ():
Nigrostriatal Dopaminergic Neurodegeneration in the Weaver Mouse Is Mediated via Neuroinflammation and Alleviated by Minocycline Administration
J. Neurosci. Peng et al.
26: 11644
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. Baculoviral p35 does not protect against Girk2wv-associated neurodegeneration in either the A8 or the A9. (A) Photomicrographs of coronal sections through the ventral midbrain illustrating localization of TH (red) and p35 (green, barless arrow in insert) proteins and their co-localization (yellow, barred arrow in insert) in the A8 and A9 of P24 +/+ and wv/wv p35 transgenic mice. Original magnification, 20×; insert magnification, 40×. (B) Photomicrographs illustrating TH-immunostaining in the A8 and the A9 of +/+ and wv/wv mice in the absence or presence of p35. Original magnification, 10×. SNpc, substantia nigra pars compacta; SNpr, substantia nigra pars reticulata; VTA, ventral vegmental area. (C) Quantitative stereological analysis of the number of TH-positived neurons from +/+, wv/wv, and wv/wv + p35 mice. Mean ± S.E.M., n = 4-5. #, p < 0.01 significantly different from +/+. (D) Quantification of striatal DA, DOPAC, and HVA levels in +/+, wv/wv, and wv/wv + p35 mice. Mean ± S.E.M., n = 3-6. #, p < 0.01 significantly different from +/+. (E) An example of a non-apoptotic degenerating silver-stained neuron in the A9 of a P24 wv/wv mouse. (F) Quantitative analysis of the number of such silver-stained degenerating cells in the A9 from wv/wv and wv/wv + p35 mice. Mean ± S.E.M., n = 4.
- supplemental material
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Supplemental Figure 2. Caspase-3 activation occurs in the weaver cerebellum but not the SNpc. Activated caspase-3 immunocytochemistry was performed using the DAB enhancement technique and sections were counterstained with hematoxylin.
