The Journal of Neuroscience, November 22, 2006, ():
Reversible Disruption of Dynactin 1-Mediated Retrograde Axonal Transport in Polyglutamine-Induced Motor Neuron Degeneration
J. Neurosci. Katsuno et al.
26: 12106
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supple Fig. 1. Neurofilament protein in SBMA mouse. A and B. Immunoblotting for phosphorylated and non-phosphorylated high molecular weight neurofilament protein in the ventral roots, sciatic nerves, and spinal cords of wildtype and AR-97Q mice (#7-8) (13-week-old). C. Anti-phospho NF-H immunohistochemistry of the ventral root and sciatic nerve from wildtype and AR-97Q mice (#7-8) (13-week-old).
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Supple Fig. 2. Retrograde labeling and anti-dynactin1 staining of spinal motor neurons in SBMA mice. Retrograde labeling of spinal motor neurons with Fluoro-gold in AR-97Q (#4-6) and wildtype mice (12 weeks). The same sections then immunostained with anti-dynactin1 antibody. The neurons with reduced Fluoro-gold labeling demonstrate low levels of dynactin1 in AR-97Q mice.
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Supple Fig. 3. Effect of histone deacetylase inhibition on dynactin1 expression in spinal cord. Immunohistochemistry of the spinal cord and skeletal muscle from sodium butyrate-treated and non-treated AR-97Q mice (#4-6, 12 weeks). Sodium butyrate treatment restores dynactin1 level in the spinal motor neurons and resolves intramuscular accumulation of neurofilament.
