The Journal of Neuroscience, November 22, 2006, ():
Intracellular and Trans-Synaptic Regulation of Glutamatergic Synaptogenesis by EphB Receptors
J. Neurosci. Kayser et al.
26: 12152
Supplemental Data
Files in this Data Supplement:
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Supplemental Figure 1. siRNA knock-down of EphB2 in neurons. Live cell images of 5DIV cultured mouse cortical neurons transfected using the calcium phosphate method. (a,b) Cultured cortical neuron transfected with expression constructs encoding EphB2-YFP (a), DsRed-2 (b), and an siRNA targeting P53 (pSuper based vector). (c) Merge of (a) and (b). (d,e) As in (a) and (b), but now with siRNA_EphB2.1 targeting EphB2. (f) Merge of (d) and (e). EphB2-YFP appears punctate and dendritically localized in (a). In contrast, expression of siRNA targeting EphB2 prevents the synthesis of detectable EphB2-YFP (d). Scale bar, 20 μm.
