The Journal of Neuroscience, November 22, 2006, ():
Somatodendritic Kv7/KCNQ/M Channels Control Interspike Interval in Hippocampal Interneurons
J. Neurosci. Lawrence et al.
26: 12325
Supplemental Data
Files in this Data Supplement:
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Manuscript Appendix
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Figure S1. Expression of (A-B) Kv7.2 and (C-D) Kv7.3 in SR/SLM in slices from GIN transgenic mice. (A) GFP expression and (B) Kv7.2+ immunoreactivity reveal colocalization of Kv7.2 in GFP+ SR interneurons (arrows) but a relative absence of Kv7.2 labeling in SLM, where axons from GFP+ interneurons terminate. (C) GFP+ SR interneurons also colocalize with (D) Kv7.3 immunoreactivity. Similarly, Kv7.3 immunoreactivity is largely absent from SLM. Scale bars: 100 µm.
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Figure S2. Linopirdine and XE-991 do not block recombinant Kv3.1/Kv3.2 channels in HEK293 cells. (A) Plot of raw steady-state Kv3.1-mediated outward current amplitudes elicited via 100 mV, 1 s long voltage steps from –60 mV at 15 s intervals during application of 20 µM XE-991, followed by 1 mM 4-AP, as indicated by the bars. (B) Averages of 3 raw traces taken at the end of (1) control, (2) 20 µM XE-991, and (3) 1 mM 4-AP periods, respectively. (C) Time series for a population of 3 cells in which 20 µM XE-991 was applied. (D) Plot of raw steady-state outward current amplitudes in which 20 µM linopirdine was applied to Kv3.2-containing cells. (E) Averages of 3 raw traces taken at the end of control (1), 20 µM linopirdine (2), and 1 mM 4-AP (3) periods, respectively. (F) Time series for a population of 6 cells in which 20 µM linopirdine was applied. (G) Plot of % of control outward current which 20 µM linopirdine (n=7) or 20 µM XE-991 (n=4); circles and squares denote recordings from Kv3.1 or Kv3.2-expressing cells, respectively. An online leak subtraction protocol (P/4) was employed prior to eliciting the voltage steps.
