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The Journal of Neuroscience, November 29, 2006, 26(48):12497-12511; doi:10.1523/JNEUROSCI.3719-06.2006

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Development/Plasticity/Repair
Transplantation of Human Neural Stem Cells Exerts Neuroprotection in a Rat Model of Parkinson's Disease

Takao Yasuhara,¹ Noriyuki Matsukawa,¹ Koichi Hara,¹ Guolong Yu,¹ Lin Xu,¹ Mina Maki,¹ Seung U. Kim,^2,3 and Cesario V. Borlongan^1,4

¹Department of Neurology, Medical College of Georgia, Augusta, Georgia 30912, ²Disease Research Center, Ajou University School of Medicine, Suwon 443-721, Korea, ³Division of Neurology, University of British Columbia Hospital, Vancouver, British Columbia, Canada V5Z 1M9, and ⁴Research and Affiliations Service Line, Augusta Veterans Affairs Medical Center, Augusta, Georgia 30904

	Abstract

Top Abstract Introduction Materials and Methods Results Discussion References

 
Neural stem cells (NSCs) possess high potencies of self-renewal and neuronal differentiation. We explored here whether transplantation of human NSCs cloned by v-myc gene transfer, HB1.F3 cells, is a feasible therapeutic option for Parkinson's disease. In vivo, green fluorescent protein-labeled HB1.F3 cells (200,000 viable cells in 3 µl of PBS) when stereotaxically transplanted (same-day lesion-transplant paradigm) into the 6-hydroxydopamine-lesioned striatum of rats significantly ameliorated parkinsonian behavioral symptoms compared with controls (vehicle, single bolus, or continuous minipump infusion of trophic factor, or killed cell grafts). Such graft-derived functional effects were accompanied by preservation of tyrosine hydroxylase (TH) immunoreactivity along the nigrostriatal pathway. Grafted HB1.F3 cells survived in the lesioned brain with some labeled with neuronal marker mitogen-activated protein 2 and decorated with synaptophysin-positive terminals. Furthermore, endogenous neurogenesis was activated in the subventricular zone of transplanted rats. To further explore the neuroprotective mechanisms underlying HB1.F3 cell transplantation, we performed cell culture studies and found that a modest number of HB1.F3 cells were TH and dopamine and cAMP-regulated phosphoprotein 32 positive, although most cells were nestin positive, suggesting a mixed population of mature and immature cells. Administration of the HB1.F3 supernatant to human derived dopaminergic SH-SY5Y cells and fetal rat ventral mesencephalic dopaminergic neurons protected against 6-hydroxydopamine neurotoxicity by suppressing apoptosis through Bcl-2 upregulation, which was blocked by anti-stem cell factor antibody alone, the phosphatidylinositol 3-kinase/Akt inhibitor LY294002 [2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one] alone, or a combination of both. These results suggest that HB1.F3 cell transplantation exerts neuroprotective effects against dopaminergic depletion in vitro and in vivo because of trophic factor secretion and neuronal differentiation.

Key words: apoptosis; neurogenesis; neuroprotection; neurodegeneration; stem cell factor; trophic

	Introduction

Top Abstract Introduction Materials and Methods Results Discussion References

 
Parkinson's disease (PD) is characterized by an extensive loss of dopamine neurons in the substantia nigra pars compacta (SNc) and their terminals in the striatum (Hornykiewicz, 1973; Calne, 1984) and affects >500,000 people in the United States (Alexi et al., 2000). The current standard of treatment for PD is oral L-3,4-dihydroxyphenylalanine, which provides symptomatic relief but does not retard the disease progression (Lang and Lozano, 1998a,b).

Neural transplantation of fetal ventral mesencephalic (VM) dopaminergic neurons has been introduced in the clinic as an alternative treatment for PD (Lindvall et al., 1988, 1992; Madrazo et al., 1988; Freeman et al., 1995; Kordower et al., 1995; Borlongan et al., 1999; Borlongan, 2000). However, recent clinical trials have generated mixed results in that transplantation of fetal VM cells was shown to induce dyskinesia in some patients (Freed et al., 2001; Olanow et al., 2003), whereas excellent graft dopaminergic reinnervation of the parkinsonian affected striatum accompanied positive clinical improvements in transplanted patients who did not develop dyskinesia (Mendez et al., 2005). Optimizing the graft material, among several other technical factors, is likely to improve the outcome and avoid adverse effects of fetal VM grafts.

Logistical issues, in particular the difficulty in obtaining ample supply of fetal VM cells coupled with their poor graft survival, limit the clinical applicability of fetal dopamine cell transplants (Borlongan and Sanberg, 2002; Freed, 2002). Finding an unlimited source of dopamine cells would be a welcome advance in cell transplant therapy. The infinite self-renewal capacity and neuronal differentiation potential of stem/progenitor cells make them an appealing donor graft source for cell therapy in PD (Snyder and Olanow, 2005; Sonntag et al., 2005). Candidate stem/progenitor cells for transplantation therapy in PD have been evaluated using several criteria, including their in vitro and in vivo phenotypic expression of the dopamine synthetic enzyme tyrosine hydroxylase (TH), as well as markers for dopamine transporter, their capacity to synthesize, release, take up, and catabolize dopamine, and also their ability to project axons to target nuclei such as the putamen (Arenas, 2002; Bjorklund et al., 2002; Lindvall and Bjorklund, 2004; Langston, 2005; Takagi et al., 2005). Although there is absent or minimal detection of fully mature neuronal differentiation of stem/progenitor cells into dopaminergic neurons, attenuation of parkinsonian symptoms have been observed, possibly as a result of the secretion of neurotrophic factors by the cells(Jung et al., 2004; Goldman, 2005; Rafuse et al., 2005).

In this study, we evaluated human HB1.F3 neural stem cells (NSCs) as graft source for PD. Initially, we transplanted HB1.F3 cells into the striatum of 6-OHDA-lesioned rats and assessed whether the cells produced behavioral benefits and protected against dopaminergic depletion. To reveal mechanisms underlying the therapeutic outcome, we subsequently characterized the neuronal phenotype of grafted HB1.F3 cells and their potential to activate the endogenous neurogenesis, especially in the subventricular zone (SVZ) adjacent to the transplant site. In addition, cultured human dopaminergic SH-SY5Y cells and fetal rat ventral mesencephalic dopaminergic cells were used to examine secretion of neurotrophic factors by HB1.F3 cell grafts.

	Materials and Methods

Top Abstract Introduction Materials and Methods Results Discussion References

 
In vivo study Production of green fluorescent protein–HB1.F3 cells. HB1.F3 cells, a human fetal-derived neural stem cell line, as described previously in detail, were kindly provided by Dr. Seung U. Kim (University of British Columbia, Vancouver, British Columbia, Canada) (Flax et al., 1998; Cho et al., 2002; Kim, 2004). Briefly, primary dissociated cell cultures from the periventricular region of human telencephalic tissues of 14 weeks gestation were prepared and grown for 10 d. Thereafter, cells were infected with an amphotropic, replication-incompetent retroviral vector-containing v-myc, and, subsequently, HB1.F3 cells were cloned. For easy detection of possible migration of transplanted cells, HB1.F3 cells were green fluorescent protein (GFP) labeled by lentivirus (Klages et al., 2000; Lundberg et al., 2002), which were kindly provided by Prof. Didier Trono (University of Geneva, Geneva, Switzerland). Briefly, pWPT–GFP as a transfer vector, pMDL g/pRRE and pRSV-Rev as packaging plasmid, and pMD2.G as an envelope plasmid were transfected to HEK293T cells (Invitrogen, Carlsbad, CA). The supernatant with viruses was collected twice for 2 d, and 1 x 10⁷ transducing units/ml of viruses were transfected to HB1.F3 cells. Morphological changes were monitored by phase-contrast microscopy (Nikon, Tokyo, Japan). Furthermore, to demonstrate the neuronal phenotypes, the degree of maturation, and the differences between GFP–HB1.F3 cells and non-GFP–HB1.F3 cells, immunocytochemical investigations were performed as described below with antibodies directed against human nuclei (mouse monoclonal IgG, 1:500; Chemicon, Temecula, CA), nestin (mouse monoclonal IgG, 1:500; Chemicon), microtubule-associated protein 2 (MAP2) (rabbit polyclonal IgG, 1:500; Chemicon), -tubulin III (mouse monoclonal IgG, 1:500; Chemicon), HuC (rabbit polyclonal IgG, 1:500; Chemicon), HuD (rabbit polyclonal IgG, 1:500; Chemicon), neuronal-specific nuclear protein (NeuN) (mouse monoclonal IgG, 1:200; Chemicon), TH (rabbit polyclonal IgG, 1:500; Chemicon), dopamine and cAMP-regulated phosphoprotein 32 (DARPP32) (rabbit polyclonal IgG, 1:500; Abcam, Cambridge, MA), and choline acetyltransferase (ChAT) (goat polyclonal IgG, 1:500; Chemicon) with secondary antibodies [goat anti-mouse/anti-rabbit/anti-goat IgG Alexa Fluor 594 and bisBenzimideH 33342 trihydrochloride (Hoechst33342; 1:1000; Sigma, St. Louis, MO)]. For estimation of neuronal marker expression, randomly selected visual fields were photographically captured (Axiophot2; Zeiss, Oberkochen, Germany), and cells were quantified by counting per high-power field view also selected at random (n = 6 in each group, 28,800 µm²). Control studies involved exclusion of primary antibody substituted with 10% normal horse serum in PBS. No immunoreactivity was observed in these controls.

Subjects and surgical procedures. We used 72 Sprague Dawley rats (200–250 g at the beginning of the experiment; Harlan Sprague Dawley, Indianapolis, IN) according to the approved guidelines of the institutional animal care and use committee of Augusta Veterans Affairs Medical Center. They were housed singly in a temperature and humidity controlled room that was maintained on 12 h light/dark cycles, with access to food and water ad libitum. All surgical procedures were conducted under aseptic conditions. Rats were anesthetized using Equithesin (300 mg/kg, i.p.) and received 6-OHDA injection (8 µg/3 µl in saline containing 0.2 mg/ml ascorbic acid; Sigma) in the right medial forebrain bundle [target coordinates: anteroposterior (AP), –4.0; mediolateral (ML), 0.8; dorsoventral (DV), 8.0 mm from bregma] using a Hamilton syringe (26 gauge needle). Immediately after the lesion surgery, 32 rats received ipsilateral intrastriatal transplantation of GFP–HB1.F3 cells (200,000/3 µl in PBS) (target coordinates: AP, 0.5; ML, 3.0; DV, 5.0 mm from bregma) was performed using a stereotaxic instrument (David Kopf Instruments, Tujunga, CA). These transplanted rats were killed at selected time points (day 3, week 1, week 2, or week 4 after transplantation) to reveal graft survival over time. A total of 40 rats (n = 8 per group) served as controls as follows: vehicle (3 µl of PBS), dead HB1.F3 cells by freeze–thaw cycle regimen (Modo et al., 2003) (200,000/3 µl in PBS), culture medium (CM) derived from HB1.F3 cells (3 µl), human recombinant stem cell factor (SCF) (500 pg/3 µl; R & D Systems, Minneapolis, MN), and continuous SCF infusion (500 pg/d) using osmotic minipumps (model 2004; Alzet, Cupertino, CA). Each control solution was stereotaxically injected into the original striatal transplant coordinates, while the osmotic minipump was intrastriatally placed targeting also the same transplant coordinates. Animals were placed on a temperature-controlled blanket until they recovered from the anesthesia. All rats received daily immunosuppression (10 mg/kg cyclosporin A, i.p.), which was started on the day of transplantation and was maintained throughout the survival period.

Behavioral testing. Spontaneous locomotor activity test, cylinder test, and apomorphine-induced rotational test were performed before transplantation and at 2 and 4 weeks after transplantation (supplemental Fig. 1A, available at www.jneurosci.org as supplemental material). For spontaneous locomotor test, each rat was placed in the Accuscan (Columbus, OH) monitor box, which was made of Plexiglas (42 x 42 x 30 cm) and equipped with infrared beams programmed to collect, over a 12 h period, hourly data of eight behavioral parameters (horizontal activity, total distance traveled, movement number, movement time, resting time, vertical activity, vertical movement number, and vertical movement time). Mean hourly data for each behavioral parameter were used for analysis.

Forelimb akinesia was analyzed with the modified cylinder test (Kirik et al., 2000; Schallert et al., 2000; Iancu et al., 2005). Briefly, rats were placed individually in a glass cylinder (21 x 34 cm) equipped with a video camera. After 10 rears, weight-bearing contacts by each forelimb on the cylinder wall were counted. Data were analyzed as percentages of left forelimb contacts over total contacts.

Rats were also challenged with apomorphine (0.3 mg/kg, s.c.; Sigma), and rotational behaviors were assessed for 60 min with a video camera. Full 360° turns contralateral to the lesion were counted.

Bromodeoxyuridine labeling. To reveal whether HB1.F3 cells activated endogenous neurogenesis in the SVZ, bromodeoxyuridine (BrdU) proliferative cells were evaluated (supplemental Fig. 1A, available at www.jneurosci.org as supplemental material). For labeling of proliferative cells (Chen et al., 2004), all rats received daily BrdU injection (50 mg/kg, i.p.; Sigma) for 2 weeks before they were killed.

Fixation and sectioning. At day 3, week 1, week 2, or week 4 after transplantation surgery, under deep anesthesia with Equithesin (500 mg/kg, i.p.), randomly selected rats were perfused through the ascending aorta with 200 ml of cold PBS, followed by 150 ml of 4% paraformaldehyde (PFA) in PBS. Brains were removed and postfixed in the same fixative for 24 h, followed by 30% sucrose in phosphate buffer for 1 week. Six series of coronal sections were cut at a thickness of 20 µm in a freezing microtome (Leica, Nussloch, Germany) and stored at –20°C.

Immunohistochemistry. To demonstrate graft survival, neuronal phenotype expression, and synapse formation, immunohistochemical investigations were performed. Free-floating sections were incubated overnight at 4°C with an anti-MAP2 antibody (mouse monoclonal or rabbit polyclonal IgG, 1:1000; Chemicon), anti-TH antibody (mouse monoclonal or rabbit polyclonal IgG, 1:1000; Chemicon), an anti-human nuclei antibody (mouse monoclonal IgG, 1:300; Chemicon), an anti-synaptophysin antibody (mouse monoclonal IgM, 1:500; Abcam), an anti-c-kit antibody (rabbit polyclonal IgG, 1:200; Abcam), an anti-TrkB antibody (rabbit polyclonal IgG, 1:200; Chemicon), and an anti-Doublecortin (Dcx) antibody (guinea pig polyclonal IgG, 1:1000; Abcam) with 10% normal horse serum (Vector Laboratories, Burlingame, CA) and 0.2% Triton X-100 (Fischer Scientific, Pittsburgh, PA) except for staining with anti-TrkB and c-kit antibodies. A slightly modified BrdU staining (Greisen et al., 2005) was used. Briefly, sections were incubated in HCl (1N, 37°C) for 30 min. After rinses with PBS three times, sections were incubated for 36 h at 4°C with an anti-BrdU antibody (rat monoclonal IgG, 1:200; Serotec, Raleigh, NC), 10% of normal horse serum, and 0.3% of Triton X-100. After several rinses in PBS, sections were incubated for 1 h in goat anti-mouse IgG Alexa Fluor 350/488/594 conjugate (1:500/1:500/1:1000; Invitrogen), goat anti-rabbit IgG Alexa Fluor 488/594 conjugate (1:500/1:1000), goat anti-rat IgG Alexa Fluor 488 (1:500), or donkey anti-guinea pig IgG Alexa Fluor 594 (1:1000) with Hoechst33342 (1:1000; Sigma). The sections were then washed three times in PBS and mounted on Superfrost Plus glass slides (Erie Scientific, Portsmouth, NH) and embedded with mounting medium (Biomeda, Foster City, CA). Control studies included exclusion of primary antibody substituted with 10% normal horse serum in PBS. No immunoreactivity was observed in these controls.

Morphological analysis. The degree of dopaminergic preservation, graft survival, and endogenous neurogenesis were immunohistochemically analyzed. For estimation of preservation of TH-positive fibers in the striatum and neurons in the SNc, surviving rate of transplanted cells, and BrdU/Dcx double-positive cells in the SVZ, randomly selected visual fields were photographically captured (Axiophot2; Zeiss), and cells were quantified by counting per high-power field view also selected at random (28,800 µm²) and corrected by the Abercrombie formula. Two captured fields in each coronal level, using three levels, were used to analyze the density of TH-positive fibers in the striatum (AP, 1.5, 0.5, and –0.5 mm to bregma) and TH-positive neurons in the SNc (AP, –4.8, –5.3, and –5.8 mm) (Paxinos and Watson, 1998). For analyses of the density of TH-positive fibers in the striatum, two areas of the right striatum (lesioned with or without transplantation) and the two corresponding areas in the contralateral side were analyzed using Scion Image software (Scion, Frederick, MD). Binary images were created using a distinct threshold, and then the positive areas were calculated and summed up. The ratio of the value in the lesioned striatum to the intact side was used for statistical analyses. For estimation of TH-positive neurons in the SNc, TH-positive cell bodies across three coronal levels of the SNc, using two sections per level, were counted and summed up in each level, and the percentages to the intact side were calculated. For evaluation of graft survival, GFP-labeled cells stained with human nuclei antibody and Hoechst33342 were counted every fifth 20-µm-thick coronal tissue section through the transplanted striatum. For assessment of BrdU/Dcx double-positive cells in the SVZ, three sections at bregma level were captured, and the number of double-positive cells was counted in four defined areas (200 x 60 µm) of the lateral ventricle wall (Baldauf and Reymann, 2005). Additionally, confocal analysis was performed using Zeiss LSM 510 confocal laser scanning microscope.

In vitro study Effects of HB1.F3-derived culture media on 6-OHDA neurotoxicity. To create an in vitro PD model, human dopaminergic SH-SY5Y neurons (American Type Culture Collection, Manassas, VA) were exposed to 6-OHDA (supplemental Fig. 1B, available at www.jneurosci.org as supplemental material) (Spina et al., 1992; Maruyama et al., 2001, 2002). SH-SY5Y cells were cultured at a density of 1 x 10⁴ cells per well on LAB-TEK chamber slide (Nalge Nunc, Rochester, NY) for immunocytochemical investigation or on a 96-well plate (Evergreen Scientific, Los Angeles, CA) for MTT assay (Promega, Madison, WI) in DMEM (Invitrogen) with high glucose supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 100 I.U/ml penicillin, and 100 µg/ml streptomycin (Mediatech, Herndon, VA). At 2 d after culture, cells were exposed to 1, 10, and 40 µM 6-OHDA (Sigma) or PBS for 12 or 24 h at 37°C. After this exposure, the medium was removed and the cultures were analyzed. Each treatment condition was replicated three times using three different cell cultures. After confirmation of appropriate 6-OHDA neurotoxic doses, we examined the neuroprotective effects of HB1.F3 cells in 6-OHDA-exposed SH-SY5Y cells.

Neurotrophic effects of HB1.F3 cells were explored using the CM (supplemental Fig. 1C, available at www.jneurosci.org as supplemental material). To collect the CM, HB1.F3 cells were cultured in 4 ml of medium at 1 x 10⁶ cells/25 cm² flask (Becton Dickinson, Hunt Valley, MD). At 2 d after culture, CM was exchanged to 2 ml of plain DMEM, and cells were incubated for 24 h, filtered with 0.22 µm syringe filter (Fischer Scientific), and preserved at –80°C. The number of HB1.F3 cells was counted for accurate secretory function per 10⁶ cells. Thereafter, SH-SY5Y cells were cultured as described above. At 2 d after culture, medium was exchanged to DMEM with 5% FBS as a control or mixture of DMEM with 10% FBS and preserved CM (1:1). Ten minutes later, 6-OHDA was added to the final concentration of 40 µM as per our previous studies and those of others (Maruyama et al., 2001, 2002; Yasuhara et al., 2004, 2005). At 24 h after 6-OHDA treatment, the cultures were examined using MTT assay (Promega) or immunocytochemically (see below). Each treatment condition was replicated three times using three different cell cultures.

Cell–cell interactive effects of HB1.F3 cells were also explored using coculture of fixed cells (supplemental Fig. 1D, available at www.jneurosci.org as supplemental material). Each 2 x 10³ HB1.F3 cells were plated on LAB-TEK chamber slides. At 24 h after culture, cells were fixed with 1% PFA for 1 h on ice and washed in PBS five times. This procedure preserved membrane integrity but deleted cell secretory function (Kuroiwa et al., 1999; Olsnes et al., 2002). SH-SY5Y cells were cultured at a density of 1 x 10⁴ cells per well on the chamber slide with fixed HB1.F3 cells. At 48 h after SH-SY5Y cell culture, 40 µM 6-OHDA or PBS were added to the cells. Finally, cells were fixed and analyzed immunocytochemically after another 24 h.

Cell viability was evaluated with MTT assay following the protocol of the manufacturer (Promega). Briefly, MTT assay was performed by adding 100 µl of MTT assay solution to 100 µl of medium containing cells in a 96-well plate after incubation of cells with 6-OHDA. The intensities of chemiluminescence of MTT activity were measured and calculated by Image station 2000R system (Eastman Kodak, Rochester, NY). In parallel, immunocytochemical evaluation of SH-SY5Y cells was performed as described previously (Yasuhara et al., 2005). Briefly, after fixation by 4% PFA for 20 min and washing with PBS three times, cells were incubated overnight at 4°C with TH antibody (rabbit polyclonal IgG, 1:500; Chemicon) with 10% normal horse serum (Vector Laboratories). After rinses in PBS three times, cells were incubated at room temperature for 30 min in goat anti-rabbit IgG Alexa Fluor 488 conjugate (1:1000; Invitrogen) and Hoechst33342 (1:2000; Sigma). The cells were then washed three times in PBS and embedded with mounting medium. For estimation of cell viability, randomly selected visual fields were photographically captured (Axiophot2; Zeiss), and cells were quantified by counting per high-power field view also selected at random (n = 6 in each group, 28,800 µm²). In addition, we used the following antibodies to reveal neuronal phenotypes of SH-SY5Y cells: primary antibodies included human nuclei (mouse monoclonal IgG, 1:500; Chemicon), nestin (mouse monoclonal IgG, 1:500; Chemicon), MAP2 (rabbit polyclonal IgG, 1:500; Chemicon), and -tubulin III (mouse monoclonal IgG, 1:500; Chemicon), whereas secondary antibodies consisted of goat anti-mouse/anti-rabbit IgG Alexa Fluor 488 conjugate (1:1000; Invitrogen). Control studies involved exclusion of primary antibody substituted with 10% normal horse serum in PBS. No immunoreactivity was observed in these controls.

Secretory function of HB1.F3 cells. Trophic factors such as SCF, brain derived-neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), and vascular endothelial growth factor (VEGF) have been detected as critical secretory factors in immature NSCs (Sieber-Blum, 1998; Lu et al., 2003; Llado et al., 2004). Thus, we measured these molecules as possible neurotrophic factors secreted by HB1.F3 cells. In addition to the CM of HB1.F3 cells collected as described above, SH-SY5Y cells and human-derived multipotent progenitor cells (hMPCs) (a kind gift from Athersys, Cleveland, OH) were also cultured, which served as the control cell line for evaluation of trophic factor secretion. The levels of BDNF, GDNF, VEGF, and SCF released from 1 million HB1.F3 or SH-SY5Y cells were determined using ELISA kits according to the protocols of the manufacturer (BDNF and GDNF from Promega; human VEGF and human SCF from R & D Systems). The CM of the cells was analyzed by interpolation from the standard curves assayed on individual plates. These ELISA systems can detect a minimum of 7.8, 15.6, 31.2, and 31.2pg/ml BDNF, GDNF, VEGF, and SCF, respectively. Both GFP–HB1.F3 cells and non-GFP–HB1.F3 had the same potencies to secrete specific growth factors in vitro.

Validation of dominant trophic factors secreted by HB1.F3 cells. ELISA revealed that HB1.F3 cells secreted BDNF and SCF. Next, to confirm which highly potent neurotrophic factors exerted the neuroprotective effects, the combination of the CM and anti-SCF antibody (rabbit polyclonal IgG, 10 ng/ml; Abcam) or anti-BDNF antibody (rabbit polyclonal IgG, 10 ng/ml; Abcam) was used. In addition, SCF-removed CM by immunoprecipitation (Kirkegaard & Perry Laboratories, Gaithersburg, MD) was also performed to eliminate possible effects of SCF–antibody complex (supplemental Fig. 1E, available at www.jneurosci.org as supplemental material).

Because the antibody neutralizing/removal data indicated the apparent dominance of SCF over BDNF, we proceeded to explore the direct neuroprotective effects of SCF against 6-OHDA neurotoxicity in SH-SY5Y cells. Recombinant human SCF (R & D Systems) was tested in a similar manner. Two doses of SCF (5 and 500 ng/ml) were chosen by the secreted amount of SCF in the CM of HB1.F3 and the ED₅₀ (2.5 ng/ml; information from R & D Systems). SCF was added to cultured SH-SY5Y cells using the same paradigm as above, consisting of CM treatment with or without anti-SCF antibody (supplemental Fig. 1E, available at www.jneurosci.org as supplemental material).

Mechanisms underlying SCF neuroprotection. To clarify whether neuroprotective effects of the CM with SCF involved anti-apoptotic effects, a modified method for terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling, (TUNEL) (Roche, Mannheim, Germany) was performed to evaluate apoptotic cell death, as described in our previous report (Yasuhara et al., 2004). At 12 h after 6-OHDA treatment, cultured cells with or without CM treatment were fixed with 4% PFA for 20 min and then washed with PBS (supplemental Fig. 1F, available at www.jneurosci.org as supplemental material). Nuclear staining with Hoechst33342 (1:2000) was performed at the same time, and the staining pattern of apoptotic cells was confirmed microscopically. The number of TUNEL-positive apoptotic cells was counted per high-power field view selected at random (n = 6 in each group, 288,000 µm²). Each treatment condition was replicated three times using three different cell cultures.

After confirming the involvement of anti-apoptotic effects of HB1.F3 cell-derived CM, we measured expression of c-kit, the SCF receptor, and TrkB, the BDNF receptor, on non-6-OHDA-exposed SH-SY5Y cells using antibodies against c-kit (rabbit polyclonal IgG, 1:200; Abcam) and TrkB (rabbit polyclonal IgG, 1:200; Chemicon). BDNF neuroprotection against 6-OHDA toxicity in SH-SY5Y cells has been reported previously to involve TrkB (Spina et al., 1992), but, to date, the role of c-kit expression, via which SCF might exert neuroprotection (Ashman, 1999), remains to be fully determined. We investigated here c-kit expression after exposure of SH-SY5Y cells to 1, 10, or 40 µM 6-OHDA (supplemental Fig. 1G, available at www.jneurosci.org as supplemental material). We also examined the molecular signaling pathway involved in SCF neuroprotection by treatment with LY294002 [2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one], a phosphatidylinositol 3-kinase (PI3k)/Akt inhibitor (600 ng/ml; Calbiochem, La Jolla, CA), using the same paradigm as the neutralizing antibody experiment (supplemental Fig. 1E, available at www.jneurosci.org as supplemental material). Although SCF–c-kit binding activates multiple signaling components, PI3k/Akt is the widely characterized pathway (Linnekin, 1999); thus, we focused on this signaling mechanism.

Bcl-2 expression, which directly exerts anti-apoptotic effects through Akt/PI3k pathways (Farlie et al., 1995), appears primarily affected by SCF–c-kit binding (McGill et al., 2002). Thus, Bcl-2 protein level was quantitatively measured to explore whether anti-apoptotic effects of SCF in 6-OHDA-exposed dopaminergic neurons was mediated by Bcl-2 upregulation (supplemental Fig. 1H, available at www.jneurosci.org as supplemental material). After each treatment of CM or SCF with or without anti-SCF antibody and LY294002 to 6-OHDA-exposed SH-SY5Y cells, attached cells cultured on 6 cm dishes (Falcon, Franklin Lakes, NJ) were washed in PBS and collected in radioimmunoprecipitation assay (RIPA) buffer (Sigma) with protease inhibitor cocktail (Complete mini; Roche) at 3, 6, 12, and 24 h after 6-OHDA treatment. Cell lysates were prepared for Bcl-2 ELISA following the protocol of the manufacturer (Calbiochem). Briefly, antigen extraction agent was added to the cell lysates in RIPA buffer and incubated on ice for 30 min. Lysates were centrifuged, and the supernatant was preserved at –80°C. To measure Bcl-2 protein from the same number of cells, the total amount of protein in each lysate was assayed (Biophotometer; Eppendorf Scientific, Westbury, NY). The amount of Bcl-2 protein was evaluated per equal quantity of total protein because the number of attached cells during 6-OHDA exposure decreased over time.

After the series of in vitro studies using SH-SY5Y cells, neuroprotective effects of CM and SCF were also confirmed using rat-derived ventral mesencephalic dopaminergic neurons (BrainBits, Springfield, IL) (supplemental Fig. 1I, available at www.jneurosci.org as supplemental material). In accordance with the instructions of the manufacturer, cells were cultured with the prepared medium (Neurobasal/B27/0.5 mM glutamine) on lysine-coated 96-well dishes for MTT assay (n = 4 in each condition) or chamber slides for immunocytochemical assay (n = 3 in each condition). The SH-SY5Y protocol was followed except for the initial plating duration and the dose of 6-OHDA (20 µM), determined as the "effective toxic dose" for this cell type in our preliminary studies (data not shown). In addition, c-kit expression was also explored in these dopaminergic neurons.

Statistical analysis. Secreted factors from cultured cells, cell viability, TUNEL-positive cells, and Bcl-2 expression with various treatments in vitro and immunohistochemical/behavioral data in vivo were evaluated using repeated-measures ANOVA or one-way ANOVA, followed by post hoc Scheffé's test. Statistical significance was preset at p < 0.05.

	Results

Top Abstract Introduction Materials and Methods Results Discussion References

 
In vivo study
Establishment of GFP–HB1.F3 cells
Most of the HB1.F3 cells were well stained with anti-nestin, anti--tubulin III, anti-HuNu, and anti-HuD, and some stained with anti-MAP2 and rarely stained with anti-NeuN, but none were positive for anti-HuC antibody, confirming that this cell line was human derived, with most cells expressing early neuronal progenitor phenotypic markers (Fig. 1). Some cells strongly expressed DARPP32, and a few expressed TH (non-GFP–HB1.F3, 14.2 ± 2.1 and 2.3 ± 0.3% of total cells, respectively) but not ChAT. Thus, some HB1.F3 cells had mature neuronal phenotypic markers that are routinely recognized in striatal cells (Fig. 1). HB1.F3 and GFP–HB1.F3 cells had the same morphology as evidenced by phase-contrast microscopy (Fig. 1). The doubling times of cultured HB1.F3 and GFP–HB1.F3 cells are 19.9 ± 1.7 and 19.8 ± 0.9 h, clearly indicating that GFP labeling does not affect the proliferative potencies of HB1.F3 cells. Immunocytochemical examinations also revealed the same expression pattern of neuronal markers between the cell lines [i.e., GFP–HB1.F3 cells expressed DARPP32 (14.1 ± 1.3%) and TH (2.0 ± 0.5%) but negligible ChAT, which did not significantly differ from non-GFP–HB1.F3 cells], thus suggesting that GFP labeling did not alter the phenotypic features of the cells except for GFP expression. The rate of strong GFP expression, which gradually decreased in vitro over 3 months, was 27.1 ± 1.5%, although the initial GFP expression rate was >90% (Fig. 1).

View larger version (54K): [in this window] [in a new window]   Figure 1. Phenotypic characterization of HB1.F3 cells and GFP–HB1.F3 cells in vitro. Immunocytochemical evaluation of HB1.F3 cells revealed that several cells were positive for nestin, human nuclei, HuD, and -tubulin III antibody, some stained for MAP2, and some rarely labeled with NeuN. Some cells were DARPP32 and TH positive, thus indicating that the cells display the characteristics of striatal neurons, although their morphological immature appearance suggests an early progenitor cell type. Parallel immunocytochemical evaluations of GFP–HB1.F3 cells revealed the same expression of phenotypic markers and morphology found in non-viral labeled HB1.F3 cells. A, Nestin; B, human nuclei; C, HuD; D, MAP2; E, NeuN; F, DARPP32 (red) and Hoechst33342 (blue); G, TH (red) and Hoechst33342 (blue); H, phase-contrast image of HB1.F3 cells; I, GFP–HB1.F3 cells (blue, Hoechst33342); J, -tubulin III (red); K, MAP2 (red); L, phase-contrast image of GFP–HB1.F3 cells. Scale bar, 30 µm.

 
Behavioral amelioration of HB1.F3 cell-transplanted rats
Accuscan tests demonstrated a near normal spontaneous locomotor activity in HB1.F3-transplanted rats at 2 and 4 weeks after transplantation in all eight categories, whereas a significant hypoactivity was detected in vehicle-treated, dead HB1.F3-transplanted, CM and single SCF-injected rats, but not continuous SCF delivery at both test periods (for statistics, see Table 1). Rats receiving continuous SCF infusion demonstrated partial amelioration compared with vehicle-treated rats but not the other control groups (Table 1). Cylinder test revealed that the ratio of the affected forelimb use of HB1.F3-transplanted rats (41.2 ± 3 and 41.9 ± 2.3% to total forelimb use at 2 and 4 weeks after transplantation) was significantly higher than that of vehicle-treated, dead cell-transplanted, CM and single SCF-injected rats (2 weeks, 25.8 ± 1.8, 20.0 ± 2.7, 23.7 ± 3.5, and 26.3 ± 1.8%; 4 weeks, 22.5 ± 3.9, 18.1 ± 3.0, 18.1 ± 3.8, and 19.4 ± 3.6%, respectively) but not that of continuously SCF-infused rats (28.1 ± 5.4 and 28.1 ± 5.0%). However, SCF continuous infusion did not significantly differ from the other control groups, thus indicating that forelimb akinesia was robustly ameliorated by transplanted HB1.F3 cells and only partially by osmotic minipump SCF delivery (repeated-measures ANOVA, F_(5,42) = 7.2; p < 0.0001; and post hoc t tests of p values <0.05 for both test periods) (Fig. 2A). The number of apomorphine-induced rotations of transplanted rats was significantly lower (0.86 ± 0.94 and 1.3 ± 0.52 turns/min at 2 and 4 weeks after transplantation) than that of vehicle-treated, dead cell-transplanted, CM and single SCF-injected rats (2 weeks, 5.3 ± 0.54, 5.3 ± 0.92, 5.4 ± 0.96, and 5.0 ± 0.75; 4 weeks, 5.5 ± 0.44, 6.98 ± 0.92, 6.4 ± 0.97, and 6.4 ± 0.75 turns/min, respectively) but not that of continuously SCF-infused rats (4.3 ± 0.99 and 4.3 ± 1.4 turns/min). Similar to cylinder test results, SCF continuous infusion did not significantly differ from the other control groups, thus indicating that 6-OHDA-induced dopamine receptor hypersensitivity was maximally attenuated by transplanted HB1.F3 cells but only moderately by osmotic minipump SCF delivery (repeated-measures ANOVA, F_(5,42 = 5.4; p = 0.0006; post hoc t tests of p values <0.05 for both test periods) (Fig. 2B).

View this table: [in this window] [in a new window]   Table 1. Near normal spontaneous locomotor activity in 6-OHDA-lesioned rats that received HB1.F3 cell grafts

 

View larger version (42K): [in this window] [in a new window]   Figure 2. HB1.F3 cell grafts ameliorate forelimb akinesia and apomorphine-induced rotations. A, Cylinder test revealed improved forelimb akinesia in lesioned rats that received HB1.F3 cell grafts compared with control rats, except for rats receiving continuous SCF infusion at 2 and 4 weeks after transplantation. Dashed line indicates performance of control (i.e., naive non-6-OHDA-lesioned age-matched rats). Data are shown as mean ± SE ratio of the affected forelimb use. *p < 0.05 versus control rats except for rats receiving continuous SCF infusion. B, The number of apomorphine-induced rotations in lesioned rats that received HB1.F3 cell grafts significantly decreased compared with control rats, except for rats receiving continuous SCF infusion at 2 and 4 weeks. Dashed line indicates performance of control, nonlesioned rats. Data are shown as mean ± SE rotations per minute. *p <0.05 versus control rats, except for rats receiving continuous SCF infusion.

 
Preservation of nigrostriatal dopaminergic system and expression of c-kit and TrkB in the striatum
TH staining of the striatum and the SNc, analyzed in three different coronal section levels, demonstrated that the dopaminergic nigrostriatal system of the HB1.F3-transplanted rats was significantly preserved in all levels examined (SNc, F_(5,42) = 130, p < 0.0001, p values <0.05, 54.5 ± 4.0, 48.9 ± 3.4, and 57.8 ± 3.4% to the intact side at the level of –4.8, –5.3, and –5.8 mm to bregma; striatum, F_(5,42) = 45, p < 0.0001, p values <0.05, 36.6 ± 2.4, 42.7 ± 1.8, and 28.9 ± 1.7% to the intact side at the level of 0.5, –0.5, and –1.5 mm to bregma) compared with that of all the other control rats (vehicle SNc, 4.8 ± 0.7, 5.1 ± 0.7, and 16.7 ± 2.3%, striatum, 21.1 ± 1.6, 17.6 ± 2.2, and 8.4 ± 1.1%; dead HB1.F3 SNc, 4.4 ± 0.4, 4.7 ± 0.5, and 15.9 ± 1.7%; striatum, 17.2 ± 1.5, 15.4 ± 1.5, and 9.0 ± 0.8%; CM SNc, 4.3 ± 0.4, 4.3 ± 0.6, and 16.1 ± 1.6%, striatum, 16.6 ± 1.3, 13.6 ± 2.0, and 9.2 ± 1.4%; single SCF SNc, 5.4 ± 0.6, 4.8 ± 1.0, and 16.2 ± 1.7%, striatum, 17.9 ± 2.1, 13.0 ± 1.9, and 8.3 ± 1.0%; continuous SCF SNc, 28.4 ± 3.5, 19.5 ± 2.1, and 32.0 ± 3.0%; striatum, 27.3 ± 2.8, 24.0 ± 3.2, and 15.7 ± 2.3%) (Fig. 3A–G). In addition, rats receiving continuous SCF infusion displayed significant preservation of nigrostriatal systems compared with vehicle-treated, dead HB1.F3-transplanted, CM and single SCF-injected lesioned rats (p values <0.05). The expression of the SCF receptor c-kit and the BDNF receptor TrkB on TH-positive fibers in the striatum was also confirmed immunohistochemically by confocal microscopy (Fig. 3H–M).

View larger version (77K): [in this window] [in a new window]   Figure 3. HB1.F3 cell grafts preserve TH-positive fibers in the striatum and neurons in the SNc and normalize c-kit/TrkB expression in the striatum. A–F, TH-positive fibers in the striatum and neurons in the SNc of rats that received HB1.F3 cell grafts were markedly preserved (C, F) compared with vehicle-treated rats (B, E). Intact striatum (A) and intact SNc (D) show normal TH-positive fibers and neurons, respectively. Insets are representative higher magnifications of TH-positive cells in D–F. Scale bar: A–C (captured by confocal microscopy), 50 µm; D–F, 100 µm; insets in D–F, 10 µm. G, Left, TH-positive fibers at three different coronal levels in the striatum were analyzed with a computerized image analysis system. Lesioned rats transplanted with HB1.F3 cells exhibited significantly more TH-positive fibers than all other lesioned rats. Rats with continuous SCF infusion also demonstrated significantly more TH-positive neurons than vehicle-treated, dead HB1.F3-transplanted, CM and single SCF-injected lesioned rats. Data are shown as mean ± SE values expressed as percentages relative to the intact side. *p < 0.05 versus all other rats; **p < 0.05 versus vehicle-treated, dead HB1.F3-transplanted, CM and single SCF-injected lesioned rats. G, Right, TH-positive fibers at three different levels in the striatum were analyzed with a computerized image analysis system. Lesioned rats transplanted with HB1.F3 cells demonstrated significantly more TH-positive fibers than all other lesioned rats. Rats with continuous SCF infusion also demonstrated significantly more TH-positive fibers than vehicle-treated, dead HB1.F3-transplanted, CM and single SCF-injected lesioned rats. Data are shown as mean values ± SE expressed as percentages relative to the intact side. *p < 0.05 versus all other rats; **p < 0.05 versus vehicle-treated, dead HB1.F3-transplanted, CM and single SCF-injected lesioned rats. H–M, The expression of the SCF receptor c-kit and the BDNF receptor TrkB was confirmed immunohistochemically in the intact and transplanted striatum by confocal microscopy. Yellowish fibers indicate colabeling of TH-positive fibers (green) with one of the receptors (red). The expression of both receptors in the striatum of vehicle-treated lesioned rats was difficult to identify because of severely reduced TH-positive fibers. H–J, TH (green) and c-kit (red). K–M, TH (green) and TrkB (red). H and K correspond to intact striatum, I and L represent vehicle-treated lesioned striatum, and J and M are from HB1.F3-transplanted lesioned striatum. Scale bar. 30 µm

 
Graft survival with MAP2 immunoreactivity and synapse formation
Graft survival rates at day 3, week 1, week 2, and week 4 after transplantation are 11.2 ± 1.4, 4.7 ± 0.6, 1.7 ± 0.2, and 1.1 ± 0.2%, respectively. Many GFP/human nuclei double-positive cells remained at the transplanted site at day 3 after transplantation which immunostained positively with nestin or MAP2 but rarely TH (Fig. 4A). Most cells remained at the transplanted site at 4 weeks after transplantation, characterized as a cluster with GFP expression. Some transplanted cells expressed MAP2, although there were no TH-positive transplanted cells in the striatum at this time point. Furthermore, some GFP-labeled cells were decorated with synaptophysin (Fig. 4B–D). As noted above, we acknowledge that GFP expression in vitro wanes over time, raising the possibility that some of the transplanted cells that eventually expressed weak or lack GFP expression were not included in our counts of cell survival. However, our cell count strategy reflects a conservative approach in that only cells with strong GFP expression were included. Because a dynamic (good to moderate to low) GFP-positive graft survival over transplant maturation periods is recognized, such conservative cell count approach closely approximates the general graft survival trend of this cell line.

View larger version (73K): [in this window] [in a new window]   Figure 4. HB1.F3 cell grafts survive in the lesioned striatum and enhance neurogenesis in the SVZ. A, Confocal microscopy revealed that many transplanted GFP-positive cells were well stained with anti-human nuclei antibody (A1) with nestin (A2), MAP2 (A3), and rarely TH expression (A4) at 3 d after transplantation. Scale bar, 10 µm. B–D, Almost all of the transplanted HB1.F3 cells were found at the original implantation site at 4 weeks after transplantation and positively stained with anti-human nuclei antibody (B2) and Hoechst33342 (B3) with modest GFP expression (B1; B4, triple-merged image). Some GFP-labeled HB1.F3-transplanted cells (C1) were MAP2 positive (C2; C3, merged image; C4, high magnification). In addition, some GFP-labeled HB1.F3 cells (D1) were decorated with synaptophysin-positive terminals (D2), suggesting synapse formation (D3, merged image; D4, high magnification). Scale bar: C4, D4, 6 µm; all other panels, 30 µm. The images in C and D were captured using a confocal microscope. E–G, BrdU staining of the SVZ of the intact side (E), vehicle-treated lesioned side (F), and transplanted lesioned side (G) revealed enhanced neurogenesis induced by HB1.F3 cell transplantation. Scale bar, 80 µm. H, I, Many newly formed cells (BrdU, green) in the SVZ of lesioned rats that received HB1.F3 cell grafts, but not those from vehicle-treated lesioned rats, were doublecortin (red) colabeled (yellow) (H, vehicle-treated rats; I, HB1.F3 cell-transplanted rats). Scale bar, 30 µm.

 
Enhanced neurogenesis in the SVZ
To test our hypothesis that HB1.F3 cell graft-mediated neurogenesis participated in the observed functional recovery, we performed BrdU injection from 2 weeks up to 4 weeks after lesion, corresponding to the timing of behavioral improvement. BrdU/Dcx double-positive cells were counted in the SVZ adjacent to the transplanted site to demonstrate neurogeneic activity of HB1.F3 cell grafts. The number of double-positive cells in the SVZ of the HB1.F3 cell-transplanted rats was significantly increased (142 ± 6.9% of the intact side) compared with that in vehicle-treated and CM-injected lesioned rats (122 ± 2.2 and 120 ± 6.9%, respectively; F_(5,42) = 4.4; p = 0.0027; p values <0.05) (Fig. 4E–I) but not that in dead HB1.F3-transplanted, single SCF and continuous SCF-treated lesioned rats (123 ± 3.2, 128 ± 0.6, and 134 ± 5.6%, respectively). These in vivo results demonstrate neuroprotective and neurogeneic effects of transplanted HB1.F3 cells. We then pursued in vitro experiments to further reveal the mechanisms underlying these therapeutic benefits of HB1.F3 cells.

In vitro study
Neuroprotection by HB1.F3-derived culture media against 6-OHDA neurotoxicity
Dose- and time-dependent 6-OHDA neurotoxicity to SH-SY5Y cells was confirmed by immunocytochemical investigations and MTT assay. MTT assay revealed that cells with 6-OHDA exposure for 24 h displayed more reduced MTT activity in a dose-responsive manner than those with 6-OHDA exposure for 12 h (repeated-measures ANOVA; F_(3,26) = 275; p < 0.0001; 12 h, 88.2 ± 4.4, 70.4 ± 3.1, and 10.3 ± 1.3%; 24 h, 82.7 ± 5.2, 43.9 ± 3.9, and 3.8 ± 0.6% of the untreated control with 1, 10, and 40 µM 6-OHDA, respectively; p values <0.01) (Fig. 5A). Furthermore, the number of surviving cells against 6-OHDA exposure for 24 h detected by TH/nuclear staining were significantly more than that with 6-OHDA exposure for 12 h (repeated-measures ANOVA; F_(3,20) = 114.5; p < 0.0001) in a dose-responsive manner (12 h, 86 ± 5, 59 ± 4.7, and 26 ± 1.7%; 24 h, 81 ± 4, 38 ± 3.5, and 7 ± 0.8% of the untreated control with 1, 10, and 40 µM 6-OHDA, respectively; p values <0.01) (Fig. 5B). Based on these present results and those from previous studies (Maruyama et al., 2001, 2002; Yasuhara et al., 2004, 2005), 40 µM 6-OHDA for 24 h was used in subsequent studies. In addition, immunocytochemical evaluation of SH-SY5Y cells revealed that most cells were well stained with antibodies against MAP2, TH, and human nuclei and rarely stained with anti-nestin antibody, indicating the dopaminergic neuronal phenotype of this cell line (Fig. 5C–E).

View larger version (33K): [in this window] [in a new window]   Figure 5. Neuroprotective effects of HB1.F3 cell-derived CM against 6-OHDA in cultured SHSY5Y cells. A, B, Neurotoxicity of 6-OHDA was confirmed by MTT assay and immunocytochemical (i.e., TH) evaluation. Exposure of SH-SY5Y cells to 10 or 40 µM 6-OHDA resulted in a significant reduction in cell viability. As expected, 6-OHDA exposure for 24 h induced significantly more cell loss than that seen with 12 h treatment. Data are shown as percentages ± SE of cell viability relative to the untreated control. *p < 0.01 versus untreated SH-SY5Y cells (24 h). C–E, Immunocytochemical investigation revealed that SH-SY5Y cells used in this study were MAP2- and TH-positive neurons with rare nestin expression, indicating the neuronal phenotypic maturity of the cells (C, nestin; D, MAP2; E, TH). Scale bar, 30 µm. F, G, Treatment with HB1.F3-derived CM significantly blocked the 6-OHDA-induced reduction of cell viability in SH-SY5Y cells. In addition, CM treatment exhibited a tendency to increase the proliferative capacities of SH-SY5Y cells that were not exposed to 6-OHDA. Data are shown as percentages ± SE of cell viability relative to the untreated control. *p < 0.01 versus 6-OHDA-exposed SH-SY5Y cells without CM treatment. H–K, TH staining revealed many surviving 6-OHDA-exposed SH-SY5Y cells because of CM treatment (H, no treatment; I, CM only; J, 6-OHDA only; K, 6-OHDA with CM). Scale bar, 30 µm.

 
We then proceeded to explore the neuroprotective effects of the CM against 6-OHDA toxicity. MTT activity of 6-OHDA-exposed SH-SY5Y cells treated with the CM was significantly higher than those cells without CM treatment (repeated-measures ANOVA; F_(1,13) = 253; p < 0.0001; with and without CM treatment, 53 ± 6.8 and 5.2 ± 0.8% of the untreated control; p values <0.01) (Fig. 5F). In addition, there was a tendency to increase MTT activity of CM-treated SH-SY5Y cells without 6-OHDA exposure compared with the untreated control (p values = 0.0751). Immunocytochemical results supported the MTT data, in that the number of surviving CM-treated SH-SY5Y cells was significantly higher than that of non-CM-treated cells after 6-OHDA exposure (repeated-measures ANOVA; F_(1,10) = 10.3; p = 0.0093; with and without CM treatment, 20 ± 2.4 and 7.1 ± 0.8% of the untreated control; p values <0.01) (Fig. 5G). In addition, the number of CM-treated SH-SY5Y cells without 6-OHDA exposure tended to increase compared with the untreated control (p values = 0.0904) (Fig. 5G–K).

In contrast, coculture of fixed HB1.F3 cells did not demonstrate any detectable neuroprotective effects in 6-OHDA-exposed SH-SY5Y cells (F_(1,10) = 2.8; p < 0.01; with and without coculture, 11.3 ± 1.7 and 10.4 ± 1.1% relative to untreated control; p values = 0.375), suggesting that HB1.F3 cells exerted neuroprotective effects via a mechanism that did not involve cell-to-cell contact.

Secretion of SCF and BDNF from HB1.F3 cells in vitro
The lack of evidence implicating a cell-to-cell contact-mediated neuroprotection prompted us to examine the mechanism of neurotrophic factor secretion by HB1.F3 cells. ELISA revealed that secretion of SCF and BDNF from HB1.F3 cells were 194.2 ± 8.4 and 28.9 ± 1.9 pg/10⁶cells per day, significantly higher than those from SH-SY5Y cells, 0.1 ± 0.1 and 1.9 ± 0.3 pg/10⁶cells per day (n = 10 in each group; p < 0.001). However, GDNF and VEGF were not detected in the CM of HB1.F3 and SH-SY5Y cells. The CM from hMPCs exhibited significantly larger amount of VEGF (2.74 ± 0.22 ng/10⁶cells per day) and SCF (248 ± 11.8 pg/10⁶cells per day) with a modest amount of BDNF (4.74 ± 1.1 pg/10⁶cells per day) but negligible GDNF.

Neutralization and immunoprecipitation of SCF block neuroprotection
To confirm which trophic factor in the CM plays an important role in the neuroprotection, anti-SCF and anti-BDNF antibodies were used. Anti-SCF antibody or SCF removal by immunoprecipitation, but not anti-BDNF antibody, significantly blocked the neuroprotective effects of the CM in that significantly low MTT activities were detected in these 6-OHDA-exposed cells treated with CM plus the anti-SCF antibody (ANOVA; F_(10,55) = 195; p < 0.0001; p values <0.01) (Table 2). Immunocytochemical results mimicked the MTT data as evidenced by significantly low number of surviving 6-OHDA-exposed cells treated with both the CM and anti-SCF antibody or SCF-removed CM by immunoprecipitation (ANOVA; F_(10,55) = 169; p < 0.0001; p values <0.01) (Table 2).

View this table: [in this window] [in a new window]   Table 2. Neuroprotective effects of cultured media derived from HB1.F3 cells

 
Direct neuroprotective effects of SCF against 6-OHDA neurotoxicity
With the demonstration of anti-SCF antibody neutralizing the neuroprotective effects of the CM harvested from HB1.F3 cells, we next assessed the direct neuroprotective effects of SCF itself. MTT assay revealed the neuroprotective effects of recombinant human SCF in 6-OHDA-exposed SH-SY5Y cells, which was completely neutralized by anti-SCF antibody (ANOVA; F_(6,49) = 326; p < 0.0001). Treatment with 500 pg/ml and 5 ng/ml SCF in 6-OHDA-exposed cells resulted in cell viability of 27.7 ± 2.4 and 26.1 ± 2.1%, respectively, relative to untreated control, versus 10.4 ± 2.8 and 7.5 ± 1.6% in 6-OHDA-exposed untreated cells and 6-OHDA-exposed cells treated with both SCF and anti-SCF antibody, respectively (p values <0.05) (Fig. 6). Furthermore, immunocytochemical investigation using TH staining revealed that the number of SCF-treated 6-OHDA-exposed cells was significantly higher than that of 6-OHDA-exposed untreated cells and 6-OHDA-exposed cells with both SCF and anti-SCF antibody (ANOVA; F_(6,35) = 203; p < 0.0001; 500 pg/ml and 5 ng/ml SCF-treated 6-OHDA-exposed cells, 25.4 ± 2.0 and 21.7 ± 2.7% relative to untreated control compared with 5.0 ± 0.8 in 6-OHDA-exposed untreated cells and 4.8 ± 0.8% in 6-OHDA-exposed cells with both SCF and anti-SCF antibody; p values <0.05) (Fig. 6). There were no significant differences between 500 pg/ml and 5 ng/ml SCF treatment in both MMT assay and immunocytochemical evaluations.

View larger version (30K): [in this window] [in a new window]   Figure 6. Neuroprotective effects of SCF against 6-OHDA neurotoxicity in cultured SH-SY5Y cells. Based on the secreted amount of SCF in the CM of HB1.F3 and the ED50 (2.5 ng/ml; information from R & D Systems), two doses of SCF (500 pg/ml and 5 ng/ml) were used in this study. Both doses of SCF protected cultured SH-SY5Y cells against 6-OHDA neurotoxicity, which were negated by the administration of anti-SCF antibody. However, there were no detectable proliferative effects of SCF treatment in SH-SY5Y cells when not exposed to 6-OHDA. In addition, there were no significant differences between the two doses of SCF. LowS, 500 pg/ml SCF; HighS, 5 ng/ml SCF; aS, anti-SCF antibody. Data are shown as percentages ± SE of cell viability relative to the untreated control. *p < 0.05 versus 6-OHDA-exposed untreated SH-SY5Y cells. p < 0.05 versus 6-OHDA-exposed SH-SY5Y cells with low dose of SCF treatment.

 
Mechanisms underlying SCF neuroprotection
In addition to the participation of neurotrophic factor secretion in HB1.F3 cell neuroprotection, we examined the involvement of an anti-apoptotic mechanism. TUNEL staining revealed that CM treatment in 6-OHDA-exposed cells significantly reduced the ratio of apoptotic cells compared with untreated 6-OHDA-exposed cells (repeated-measures ANOVA; F_(1,6) = 78; p = 0.0001; 3.1 ± 0.7 and 17.4 ± 1.5% of Hoechst33342-positive cells; p values <0.001) (Fig. 7A–C), indicating that HB1.F3 cells, via secretion of neurotrophic factors (i.e., SCF) in the supernatant, exerted anti-apoptotic effects.

View larger version (21K): [in this window] [in a new window]   Figure 7. Anti-apoptotic effects of HB1.F3 cell-derived CM and c-kit and TrkB expression in 6-OHDA-exposed SH-SY5Y cells. A, Administration of the CM significantly reduced the ratio of TUNEL-positive apoptotic cells to Hoechst33342-positive viable cells after 6-OHDA exposure. Data are shown as percentages ± SE of cell viability relative to the untreated control. *p < 0.01 versus 6-OHDA-exposed untreated SH-SY5Y cells. CM, Culture media of HB1.F3 cells. B, C, TUNEL staining (green) and nuclear staining with Hoechst33342 (blue) revealed markedly decreased TUNEL-positive apoptotic (arrows) SH-SY5Y cells after 6-OHDA and CM treatment (C) compared with 6-OHDA exposure without CM treatment (B). Insets are representative higher magnifications of TUNEL-positive cells in each panel. Scale bar: 60 µm; insets, 10 µm. D–G, c-kit expression (green) and nuclear staining with Hoechst33342 (blue) demonstrated that 1 µM 6-OHDA exposure for 24 h upregulated c-kit expression. D, Untreated SH-SY5Y cells. E–G, 6-OHDA treatment at 1, 10, and 40 µM to SH-SY5Y cells. Scale bar, 60 µm. H, TrkB was strongly expressed on SH-SY5Y cells. Scale bar, 30 µm.

 
As shown in a previous report (Cohen et al., 1994), c-kit and TrkB are expressed to some extent in SH-SY5Y cells under normal conditions. In the present study, c-kit expression of 6-OHDA-exposed SH-SY5Y cells was documented immunocytochemically. c-kit expression of SH-SY5Y cells with 0, 1, 10, and 40 µM 6-OHDA exposure for 24 h was 22.8 ± 1.4, 31.7 ± 1.8, 26.8 ± 1.1, and 13.6 ± 2.4% of total cells, respectively, indicating significant upregulation of c-kit expression in SH-SY5Y cells exposed to low dose (1 µM) 6-OHDA (ANOVA; F_(3,16) = 19.8; p < 0.0001; p values = 0.0182) (Fig. 7D–G). These data indicate that 6-OHDA triggered c-kit upregulation in SH-SY5Y cells, which likely enhanced SCF–c-kit binding. In addition, the BDNF receptor TrkB was also strongly expressed on SH-SY5Y cells (Fig. 7H). Such receptor upregulations might have facilitated the neuroprotective effects of SCF and BDNF. However, it appears that TrkB–BDNF binding was not optimal in the present paradigm because BDNF was shown to be only partially involved in the neuroprotection, as revealed by the antibody-neutralizing experiments (see above, Neutralization and immunoprecipitation of SCF block neuroprotection).

In our desire to determine neuroprotective downstream pathways associated with the SCF–c-kit binding, the PI3k/Akt pathway was explored by using the PI3k/Akt inhibitor LY294002. Previously, the involvement of the PI3k/Akt pathway in SCF–c-kit binding was shown in non-neural cells (Linnekin, 1999). LY294002 treatment completely cancelled the neuroprotective effects of the CM and SCF against 6-OHDA toxicity (ANOVA; MTT assay, F_(6,41) = 50.0, p < 0.0001, p values <0.01; cell survival as revealed by TH and Hoechst33342 staining, F_(6,35) = 86.7, p < 0.0001, p values <0.01) (Fig. 8A).

View larger version (44K): [in this window] [in a new window]   Figure 8. Neuroprotective effects of CM and SCF involves the PI3k/Akt pathway and Bcl-2 upregulation. A, LY294002, a PI3k/Akt inhibitor, significantly reduced the neuroprotective effects induced by CM or SCF treatment. CM, Culture media of HB1.F3 cells; L, LY294002; aS, anti-SCF antibody; LowS, 500 pg/ml SCF. Data are shown as percentages ± SE of cell viability relative to the untreated control. *p < 0.05 versus 6-OHDA-exposed untreated SH-SY5Y cells. p < 0.05 versus 6-OHDA-exposed SH-SY5Y cells with the CM or low dose of SCF treatment. B, Cultured SH-SY5Y cells exposed to 6-OHDA and treated with CM or SCF displayed significantly higher Bcl-2 expression at 12 h after 6-OHDA exposure compared with untreated 6-OHDA-exposed cell cultures. However, 6-OHDA alone produced a transient elevation of Bcl-2 expression in untreated SH-SY5Y cells at 6 h after the neurotoxin administration. In addition, Bcl-2 upregulation in 6-OHDA-exposed cells induced by CM treatment was cancelled by anti-SCF antibody and/or PI3k/Akt inhibitor. Data are shown as units per milligram of protein ± SE. CM, Culture media of HB1.F3 cells; aS, anti-SCF antibody; L, LY294002; LowS, 500 pg/ml SCF; HighS, 5 ng/ml SCF. *p < 0.05 versus untreated SH-SY5Y cells. p < 0.05 versus 6-OHDA-exposed SH-SY5Y cells with the CM.

 
Based on the results above demonstrating anti-apoptotic effects of CM and SCF, and accumulating evidence implicating Bcl-2 expression in both SCF–c-kit binding and the PI3k/Akt pathway, Bcl-2 protein level was monitored using the lysates from SH-SY5Y cells collected at 3, 6, 12, and 24 h after 6-OHDA treatment. Repeated-measures ANOVA revealed significant treatment effects (F_(9,20) = 22.8; p < 0.0001) (Fig. 8B). Single ANOVA for each post-6-OHDA time point revealed a trend at 3 h (F_(9,20) = 2.29; p = 0.059) and significant treatment effects at 6 h (F_(9,20) = 8.463; p < 0.0001), 12 h (F_(9,20) = 25.3; p < 0.0001), and 24 h (F_(9,20) = 9.3; p < 0.0001). Pairwise comparisons revealed that Bcl-2 protein levels of CM- or SCF-treated 6-OHDA-exposed cells at 6, 12, and 24 h were significantly higher than that of untreated control (p values <0.05). There were no differences in the significant Bcl-2 upregulation induced by 500 pg/ml and 5 ng/ml SCF at all time points (p values >0.05). Antibody-neutralizing treatment conditions using anti-SCF antibody alone, LY294002 alone, or a combination of both significantly blocked the CM-mediated Bcl-2 upregulation at 6, 12, and 24 h (p values <0.05).

Neuroprotective effects of CM and SCF on 6-OHDA-exposed fetal rat-derived ventral mesencephalic dopaminergic neurons
Phenotypic characterization of the rat fetal-derived ventral mesencephalic dopaminergic neurons used in this study revealed that these cells expressed TH strongly and c-kit only moderately (Fig. 9A–D), in addition to nestin, -tubulin III, and MAP2 but not DARPP32 or GFAP expression. The relatively modest c-kit expression in these rat fetal-derived dopaminergic neurons, as opposed to the high c-kit expression detected in human SH-SY5Y cells, might limit potential neuroprotective effects of SCF on this cell line. Indeed, treatment with CM and low-dose SCF displayed full and partial (only immunocytochemical effects) neuroprotection, respectively, against 6-OHDA toxicity, which were blocked by anti-SCF antibody or SCF removal with immunoprecipitation (Fig. 9E–I) [MTT assay, repeated-measures ANOVA, F_(4,15) = 10.1, p = 0.0004; with and without CM treatment, 32 ± 5.2 and 16 ± 1.9% of the untreated control, p values <0.05 (Fig. 9I, left); immunocytochemical assay, F_(4,10) = 5.2, p = 0.015; with and without CM (SCF) treatment, 34 ± 2.1 (21.2 ± 2.0) and 4.2 ± 2.1% of the untreated control, p values <0.05 (Fig. 9I, right)].

View larger version (19K): [in this window] [in a new window]   Figure 9. Neuroprotective effects of CM and SCF on 6-OHDA-exposed fetal rat-derived dopaminergic neurons. A–D, Fetal rat-derived dopaminergic neurons expressed TH (A) and c-kit (C) (B, Hoechst33342; D, merged image). Scale bar, 30 µm. E–H, TH staining revealed many surviving 6-OHDA-exposed dopaminergic neurons from rat ventral mesencephalon because of CM and SCF treatment (E, no treatment; F, 6-OHDA only; G, 6-OHDA with CM; H, 6-OHDA with SCF). Scale bar, 30 µm. I, Left, MTT assay revealed that treatment with CM completely whereas SCF only partially blocked the 6-OHDA-induced reduction of cell viability in rat-derived dopaminergic neurons. Data are shown as percentages ± SE of cell viability relative to the untreated control. *p < 0.05 versus 6-OHDA-exposed dopaminergic neurons without treatment, with CM plus anti-SCF antibody, and SCF-removed CM by immunoprecipitation. I, Right, Immunocytochemical assay revealed that treatment with CM and SCF significantly blocked the 6-OHDA-induced reduction of cell viability in rat-derived dopaminergic neurons. Data are shown as percentages ± SE of cell viability relative to the untreated control. *p < 0.05 versus 6-OHDA-exposed dopaminergic neurons without treatment (no treatment), with CM plus anti-SCF antibody (CM), SCF-removed CM by immunoprecipitation (IP), and SCF (LowS). **p < 0.05 versus 6-OHDA-exposed dopaminergic neurons without treatment, with CM plus anti-SCF antibody, and SCF-removed CM by immunoprecipitation.

 

	Discussion

Top Abstract Introduction Materials and Methods Results Discussion References

 
We demonstrated neuroprotective effects of HB1.F3 cells using in vivo and in vitro models of PD. Intrastriatal transplantation of HB1.F3 cells immediately after 6-OHDA lesion in rats reduced parkinsonian motor symptoms and preserved TH nigral neurons and striatal fibers. At 1 month after transplantation, some grafted HB1.F3 cells expressed neuronal and synaptic markers, accompanied by enhanced neurogenesis in SVZ adjacent to the transplant site. In vitro studies further reveal neuronal lineage commitment of HB1.F3 cells and their ability to secrete a highly potent neurotrophic factor capable of suppressing apoptosis.

HB1.F3 cell grafts attenuate parkinsonian motor symptoms
Overall, the reductions in parkinsonian motor symptoms produced by HB1.F3 cell grafts are equal or more robust than those reported using fetal VM, NSCs, or genetically modified cells. The significant amelioration of hypoactivity in the present HB1.F3 cell-transplanted rats compared with vehicle-infused lesioned rats parallels our previous study demonstrating similar attenuation of abnormal spontaneous locomotor activity in 6-OHDA-lesioned animals that received vitamin D3 treatment (Wang et al., 2001). Improvement in drug-induced rotations are observed after transplantation of human or mouse embryonic stem (ES) cells (Bjorklund et al., 2002; Ben-Hur et al., 2004; Yoshizaki et al., 2004), genetically modified cells (Kang et al., 1993; Leff et al., 1998; Chen et al., 2003), or NSCs or neural progenitor/precursor cells (Svendsen et al., 1997; Armstrong et al., 2002; Sun et al., 2003; Rafuse et al., 2005; Richardson et al., 2005; Harrower et al., 2006). Cell transplantation of rat embryonic VM cells at 2 weeks after 6-OHDA lesion did not attenuate the forelimb akinesia using the cylinder test (Dowd and Dunnett, 2004). In contrast, contralateral paw use was up 25% in the HB1.F3 cell-grafted lesioned rats compared with vehicle-treated lesioned animals. The present same-day lesion transplant paradigm likely contributed to the enhanced positive outcome. Such "neuroprotective" strategy is relevant to PD because motor symptoms do not occur until 70–80% of striatal nerve terminals and 50–60% of SNc perikarya have been depleted (Bernheimer et al., 1973; Riederer and Wuketich, 1976; Bezard et al., 2001).

HB1.F3 cell grafts survive in the lesioned striatum
Totipotential stem cells from bone marrow decrease their proliferative potencies with differentiation (Wagers, 2005). Even with daily immunosuppression, graft survival of v-myc oncogene-primed HB1.F3 cells was 1% at 1 month after transplantation, which is relatively low compared with at least 5% graft survival with other neural stem/progenitor cells (Borlongan et al., 1998; Yang et al., 2004