The Journal of Neuroscience, December 6, 2006, ():
Imaging of cAMP Levels and Protein Kinase A Activity Reveals That Retinal Waves Drive Oscillations in Second-Messenger Cascades
J. Neurosci. Dunn et al.
26: 12807
Supplemental Data
Files in this Data Supplement:
- supplemental material
-
Supplemental Figure 1: Spontaneous depolarizations in retinal explants are shorter in duration and lead to smaller increases in intracellular calcium than in acutely isolated retinas.
A. Current clamp recording from a RGC in a retinal explant.
B. Summary data comparing the amplitude, inter-event interval and duration of spontaneous depolarizations in retinal explants and acutely isolated retinas. Retinal explants were isolated from P0-P1 rats and kept 24-48 hours in culture (n=8 RGCs). Acutely isolated retinas are from P2 or P7 rats (n=8 RGCs from 8 retinas). (** indicates significance p<0.001, * indicates significant p< 0.05).
C. Cumulative probability plot of the fractional change of fura-2-AM fluorescence individual cells imaged at 60x magnification. Mean amplitude of spontaneous calcium transients was calculated for RGCs from acutely isolated retinas (n=203 cells) or explants cultured for 24 hours (n=164 cells).
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