The Journal of Neuroscience, February 1, 2006, ():
The Level and Integrity of Synaptic Input Regulates Dendrite Structure
J. Neurosci. Sorensen and Rubel
26: 1539
Supplemental data
Files in this Data Supplement:
- supplemental material
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Supplemental Figure 1. (A) Schematic of a cross-section through the chick brainstem (dorsal half only) containing the initial circuit of auditory processing: the eighth nerve (VIII nerve), nucleus magnocellularis (NM) and nucleus laminaris (NL). The VIII nerve carries information from one ear to NM on one side of the brain. NM then sends bilateral projections to NL on both sides of the brain. Ipsilateral projections contact the dorsal dendrites and contralateral projections decussate in the dorsal cochlear tract and contact the ventral dendrites of NL neurons. Cutting the contralaterally projecting axons at the midline selectively deafferents the ventral dendrites of NL (see scissors in schematic). With this manipulation, the dorsal dendrites of NL remain normally innervated. NM provides the only excitatory input to NL. (B) Surface-rendered, 3D projection image of a typical NL neuron filled with dextran conjugated to Alexa-594 and imaged live using multi-photon microscopy. The dorsal (D) and ventral (V) orientation of both images is indicated by the axis at the right of the brainstem schematic. Scale bar = 20 μm.
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Supplemental Figure 2. Time-lapse movie of the stimulated dorsal dendrites that appear in Figure 9. The time of image acquisition is indicated in hours in the upper left hand corner of each image. Though images are shown for four different time points, total dendritic branch length and branch dynamics were only quantitatively compared at the initial and final time points.
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Supplemental Figure 3. Representative examples of dorsal and ventral NL dendrites imaged at 0 hr and then again after 5 hrs of exposure to the glutamate receptor blockers DNQX and AP-5. Black and white images at left were taken at 0 hr. The same dendrites imaged 5 hrs later are shown in the center. A false-colored merge of the two time points is shown on the right. Both sets of dendrites exhibit substantial branch retraction after 5 hrs of glutamate receptor blockade. Arrows indicate dendritic branches that have lost length between the initial and final imaging time points.
