The Journal of Neuroscience, December 20, 2006, ():
Brain-Derived Neurotrophic Factor Participates in Determination of Neuronal Laminar Fate in the Developing Mouse Cerebral Cortex
J. Neurosci. Fukumitsu et al.
26: 13218
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Figure S1 Detail analysis of distributional changes of BrdU positive migrating neurons in the cerebral cortex of BDNF- or vehicle-treated mice. The cerebral cortex was evenly divided into ten parts numbered from the ventricular to the pial surface, and the number of BrdU positive cells in each sector was counted. The number of cells in the indicated sector is expressed as a percentage of the total number of BrdU positive cells in the whole cortex. The SEs are not shown when they are less than the width of the symbols.
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Figure S2 Detail analysis for the positional changes of BrdU+ nuclei of cortical progenitors. The number of positive nuclei in each sector is expressed as a percentage of the total number of BrdU+ nuclei. The cerebral cortex was evenly divided into ten parts numbered from the ventricular to the pial surface, and the number of BrdU positive cells in each sector was counted. The number of cells in the indicated sector is expressed as a percentage of the total number of BrdU positive cells in the whole cortex. The SEs are not shown when they are less than the width of the symbols. Significant differences from the corresponding control (no treatment with BDNF) were determined by Student’s t-test. Significance, *P<0.05, **P<0.01, ***P<0.005 (n=4)..
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Figure S3 BDNF and its high affinity receptor, TrkB gene are expressed on the embryonic mouse cortex during the neurogenesis period. (A) Total RNAs from E13.5, 15.5, 18.5, and adult cortexes were subjected to RT-PCR using primers specific for BDNF, TrkB and β-actin. The PCR products were electrophoresed on 2% agarose gels, and the gels were stained with ethidium bromide. (B) The intensity of the target band was densitometrically quantified, and the ratio of the intensity of each stage band to that of the β-actin band was calculated. Values represent mean±SE (n=4).
