The Journal of Neuroscience, December 27, 2006, ():
Phase Precession in Hippocampal Interneurons Showing Strong Functional Coupling to Individual Pyramidal Cells
J. Neurosci. Maurer et al.
26: 13485
Supplemental Data
Files in this Data Supplement:
- supplemental material
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Supplementary Figure 1: EEG analysis for the automatic identification of ripple epochs. Top Panel: A sample of the raw EEG signal recorded during rest. Second Panel: EEG signal filtered between 100-300Hz. Third Panel: Rectified EEG data. Data was rectified by squaring the filtered signal (second panel). Bottom Panels: The rectified trace was filtered between 1- 20 Hz, and all peaks amplitudes were found. The filtered data for rest (blue) and a sample from behavior (red) are presented in the bottom traces.
- supplemental material
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Supplementary Figure 2: Procedure for the automatic identification of ripple epochs. First panel: probability density functions were generated of the peak amplitudes of the low-pass filtered ripple traces recorded during the run and sleep epochs (see Supplementary Figure 1). The inset clearly shows the difference between the distributions of ripple amplitudes for sleep (blue) and run (red) epochs. The threshold for ripple identification was defined as the maximum intersection point of these two density functions. This point was determined by subtracting the two distributions in the first panel to produce the difference distribution presented in the second panel. This distribution was interpolated with the fourth panel to find the zero crossing and the corresponding threshold for ripple identification. Once ripples were identified, the precise start and end time of each ripple epoch was determined by extending the original start and end times outwards until they intersected with the mean of the filtered trace.
- supplemental material
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Supplementary Figure 3: Procedure for finding the slope of theta phase precession as discussed in the method section. The interneuron in this example can be seen in Figure 6 of the main text. Panels A-F illustrates multiple matrix rotations of the phase by position density plot from -0.4 cm/degrees to 0.4 cm/degrees. Panel C is the rotation that best maximizes the variance while panel D is the non-rotated matrix. The bottom of the figure shows the variance as a function of position for all rotations with the examples in the above panels plotted as red dots (See Supplementary Movie 1).
